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- 库存：
大量
- 运输方式：
冻存运输
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- ATCC Number：
CRL-2590™
- 免疫类型：
kappa
- 细胞形态：
淋巴样
- 生长状态：
悬浮生长，少量贴壁
| Designations: | P-9 | ||
| Depositors: | R Lane, RL Mellgren | ||
| Isotype: | kappa | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension, with some loosely adherent cells | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Isolation: | Isolation date: 1990 | ||
| Comments: | Animals were immunized with mu-calpain isolated from human erythrocytes. Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. The antibody reacts with the large subunit of human mu-calpain.. It cross-reacts with the large subunit of rat, mouse and hamster mu-calpain.The antibody does not cross-react with human placental m-calpain [PubMed: 8702541]. The antibody can be used for immunochemical and Western blot applications. It is a useful tool for detecting and localizing the large subunit of mu-calpain. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
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| Subculturing: | Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10(5) viable cells/ml. Attached cells may be subcultured by scraping. Interval: Maintain cell density between 2 X 10(5) and 1 X 10(6) viable cells/ml. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
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| Preservation: | Freeze medium: Complete growth medium supplemented with an additional 45% fetal bovine serum and 10% DMSO Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 |
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| References: | 53533: Zhang W, et al. The major calpain isozymes are long-lived proteins. Design of an antisense strategy for calpain depletion in cultured cells. J. Biol. Chem. 271: 18825-18830, 1996. PubMed: 8702541 | ||
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