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- 详细信息
- 技术资料
- 运输方式：
冻存运输
- 免疫类型：
IgG2a
- 细胞形态：
淋巴样
- 生长状态：
悬浮生长
- ATCC Number：
HB-95™
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 库存：
大量
| Designations: | W6/32 | ||
| Depositors: | P Parham | ||
| Isotype: | IgG2a | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against HLA A, B, C | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | Animals were immunized with cells from human tonsils. Spleen cells were fused with P3X63Ag8 myeloma cells. The antibody reacts with monomorphic determinants on HLA A,B,C molecules. The antibody is cytotoxic. The antibody cross-reacts with ape, monkey and cow cells. It does not react with rabbit cells. Tested and found negative for ectromelia virus (mousepox). |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| Preservation: | Freeze medium: 95% FBS ; 5% DMSO Storage temperature: liquid nitrogen vapor phase |
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| References: | 955: Brodsky FM, Parham P. Monomorphic anti-HLA-A,B,C monoclonal antibodies detecting molecular subunits and combinatorial determinants. J. Immunol. 128: 129-135, 1982. PubMed: 6172474 956: Barnstable CJ, et al. Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens -- new tools for genetic analysis. Cell 14: 9-20, 1978. PubMed: 667938 22830: Parham P, et al. Use of a monoclonal antibody (W6/32) in structural studies of HLA- A,B,C, antigens. J. Immunol. 123: 342-349, 1979. PubMed: 87477 32767: Solomon KR, et al. The association between glycoslphosphatidylinositol-anchored proteins and hetrotrimeric G protein alpha subunits in lymphocytes. Proc. Natl. Acad. Sci. USA 93: 6053-6058, 1996. PubMed: 8650218 |
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W6/32
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