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- 2025年10月12日
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- 详细信息
- 技术资料
- 运输方式：
冻存运输
- 细胞形态：
成纤维样
- 年限：
embryo
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- ATCC Number：
CRL-1404™
- 器官来源：
胚胎
- 库存：
大量
- 生长状态：
贴壁生长
| Designations: | SC-1 | ||
| Depositors: | JW Hartley | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Mus musculus | ||
| Morphology: | fibroblast |
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| Source: | Organ: embryo | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Age: | embryo | ||
| Comments: | Initial cultures from a thawed ampule may take 10 to 12 days to become confluent. Tested and found negative for ectromelia virus (mousepox). |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended Medium Renewal: 2 to 3 times per week Remove medium, add fresh 0.25% trypsin, 0.03% EDTA for 1 to 3 minutes, remove trypsin and let the culture sit at 37C for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks. |
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| References: | 22696: Hartley JW, Rowe WP. Clonal cells lines from a feral mouse embryo which lack host-range restrictions for murine leukemia viruses. Virology 65: 128-134, 1975. PubMed: 167514 | ||
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