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- 详细信息
- 技术资料
- 生长状态：
贴壁生长
- 器官来源：
胚胎
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 细胞类型：
其他细胞类型
- 年限：
14 to 17 day gestation embryo
- 库存：
大量
- ATCC Number：
CCL-163.2™
- 细胞形态：
成纤维样
- 运输方式：
冻存运输
- 品系：
BALB/c
| Designations: | M-MSV-BALB/3T3 | ||
| Depositors: | S Aaronson | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Mus musculus | ||
| Morphology: | fibroblast |
||
| Source: | Organ: embryo Strain: BALB/c Cell Type: fibroblastMoloney murine sarcoma virus (Mo-MSV) transformed |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Tumorigenic: | Yes | ||
| Antigen Expression: | H-2d | ||
| Age: | 14 to 17 day gestation embryo | ||
| Comments: | Do not produce M-MSV. Derived from BALB/3T3 (CCL-163). |
||
| Propagation: | ATCC complete growth medium: Dulbecco's modified Eagle's medium, 90%; bovine calf serum, 10% Temperature: 37.0°C |
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| Subculturing: | Protocol: Remove medium, add fresh 0.1% trypsin for 3 to 5 minutes, remove trypsin and let the culture stand at room temperature for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 5 to 7 days. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended Medium Renewal: Every 2 to 3 days |
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| Related Products: | parental cell line:ATCC CCL-163 | ||
| References: | 22253: Aaronson SA, Rowe SP. Nonproducer clones of murine sarcoma virus transformed BALB-3T3 cells. Virology 42: 9-19, 1970. PubMed: 4318987 | ||
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M-MSV-BALB/3T3
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