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- 2025年11月22日
企业认证
- 详细信息
- 文献和实验
- 技术资料
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 生长状态：
悬浮生长
- 免疫类型：
IgG2a
- 细胞形态：
淋巴样
- 库存：
大量
- 运输方式：
冻存运输
- ATCC Number：
TIB-117™
| Designations: | PAb 101 (formerly clone 412) | ||
| Depositors: | EG Gurney | ||
| Isotype: | IgG2a | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against large T antigen (SV40) | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | Animals were immunized with the SV40 transformed 3T3 clone 4 mouse cell line (B4). Spleen cells were fused with NS-1 myeloma cells. The antibody reacts with a denaturation sensitive determinant on the large T antigen. It reacts with higher affinity than PAb 100 (ATCC TIB-115 ) but does recognized a subclass of large T antigen recognized by sera from mice bearing SV40 induced tumors. Reacts strongly by immunofluorescence with nuclei of SV40 transformed cells but not with BK virus infected human cells. The antibody precipitates large T antigen and NVT (when complexed) but not small t antigen (nor the smaller SV-80 t antigen) from extracts of SV-80 cells and SV40 infected TC-7 cells. Tested and found negative for ectromelia virus (mousepox). |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%. |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp4 cells/ml and do not allow the cell concentration to exceed 5 x 10 exp5 cells/ml. |
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| References: | 895: Gurney EG, et al. Monoclonal antibodies against simian virus 40 T antigens: evidence for distinct subclasses of large T antigen and for similarities among nonviral T antigens. J. Virol. 34: 752-763, 1980. PubMed: 6155477 901: Deppert W, et al. Monoclonal antibodies against simian virus 40 tumor antigens: analysis of antigenic binding sites, using adenovirus type 2-simian virus 40 hybrid viruses. J. Virol. 37: 478-482, 1981. PubMed: 6163870 |
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文献和实验有anti-phosphothronine 和anti-phosphotyrosine的抗体，PAb和MAb都有，但是没有anti-phosphoserine的抗体。 摘抄： Anti-Phosphothreonine (MAb) - CryptateThe anti-phosphothreonine antibody is a monoclonal antibody (clone 42H4) produced at Cell Signaling
sites. All of the ten clones grew in the non-induced tubes incubated at 30°C indicating that the I-SceI endonuclease was not expressed and that the I-SceI-CS-hmLhcgr -BAC clone and the pSC101BADγβαA-I-SceI[amp] vector could continue to propagate
1 引言 克隆(clone)：是指无性繁殖细胞系，是由单一个祖先细胞分裂繁殖而形成的一簇细胞纯系。在这个家族的所有成员中，如无突变发生，其基因是完全相同的。 1、多克隆抗体（polyclonal antibody, pAb）： 用一种包含多种抗原决定簇的抗原免疫动 物，可刺激机体多个B细胞克隆产生针对多种 抗原表位的不同抗体。所获得的免疫血清实 际上是含有多种抗体的混合物。 2、单克隆抗体（monoclonal
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