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- 2025年11月05日
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- 免疫类型：
IgG; kappa light chain
- 细胞形态：
淋巴样
- 库存：
大量
- 生长状态：
悬浮生长
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 细胞类型：
B淋巴细胞
- 相关疾病：
其他疾病
- ATCC Number：
CRL-11102™
- 运输方式：
冻存运输
| Designations: | DS-1 | ||
| Depositors: | The United States of America | ||
| Isotype: | IgG; kappa light chain | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Homo sapiens | ||
| Morphology: | lymphoblast |
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| Source: | Disease: lymphangiectasia Derived from metastatic site: pleural effusion Cell Type: B lymphocyte; |
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| Cellular Products: | surface immunoglobulin | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Receptors: | interleukin 6 (IL-6) | ||
| Antigen Expression: | HLA Class I, (100%); HLA Class II (38%); plasma cell antigen (PCA) (99%); CD2 (<5%); CD3 (<5%); CD4 -; CD5 -; CD7 -; CD8 -; CD20 -; CD30 -; CD38 -; CD45 - | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 12 D13S317: 9,12 D16S539: 9,10 D5S818: 11,14 D7S820: 8,14 THO1: 6,9.3 TPOX: 9,11 vWA: 18,19 |
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| Comments: | The DS-1 cell line was established from cells in the pleural effusion from an immunodeficient patient with intestinal lymphangiectasia. DS-1 is IL-6 dependent. |
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| Propagation: | ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate supplemented with 10 U/ml IL-6, 90%; fetal bovine serum, 10% Temperature: 37.0°C |
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| Subculturing: | 55-X ). Medium Renewal: Every 2 to 3 daysATCC Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 (5) cells/ml. Maintain cultures between 1 X 10 (5) and 2 X 10 (6) cells/ml.Recovery of this line from the frozen state is improved by using a feeder layer of irradiated MRC-5 cells ( |
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| Preservation: | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
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| Bioreactive Factors: | Growth Factors: interleukin 6 (IL-6) | ||
| References: | 22116: Bock GH, et al. Monoclonal antibody recognizing a surface molecule on a subset of antigen-stimulated T cells and on certain malignancies of T and B cell origin. US Patent 5,556,947 dated Sep 17 1996 | ||
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DS-1
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