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- 2025年11月26日
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- ATCC Number：
SCRC-1008™
- 库存：
大量
- 运输方式：
冻存运输
- 品系：
C57BL/6
- 生长状态：
贴壁生长
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 细胞形态：
成纤维样
- 器官来源：
其他
- 细胞类型：
成纤维细胞
- 年限：
14 days gestation embryo
| Designations: | MEF (C57BL/6) [MEF-BL/6-1] | ||
| Depositors: | ATCC | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Mus musculus | ||
| Morphology: | fibroblast |
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| Source: | Organ: embryo, whole Strain: C57BL/6 Cell Type: fibroblast |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Isolation: | United States Isolation date: January 22, 2003 |
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| Applications: | The cells can be used as a feeder layer to support the growth of embryonic stem (ES) cells and for the maintenance of ES cells in the undifferentiated state. The growth of these cells must be arrested before they can be used as a feeder layer. ATCC has successfully treated the cells with mitomycin C for use as a feeder layer. The cell line was established by ATCC in 2003 from dissociated C57BL/6 mouse embryos. If the MEFs are being used as a feeder layer for ES cells, it is not recommended to use them past passage no. 6 (P6). |
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| Age: | 14 days gestation embryo | ||
| Gender: | male and female mixed | ||
| Comments: | The cell line was established by ATCC in 2003 from dissociated C57BL/6 mouse embryos. The cells can be used as a feeder layer to support the growth of embryonic stem (ES) cells and for the maintenance of ES cells in the undifferentiated state. The growth of these cells must be arrested before they can be used as a feeder layer. ATCC has successfully treated the cells with mitomycin C for use as a feeder layer. If the MEFs are being used as a feeder layer for ES cells, it is not recommended to use them past passage no. 6 (P6). | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
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| Subculturing: | Protocol: Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:5 to 1:8 |
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| Preservation: | Freeze medium: Complete growth medium, supplemented with an additional 40% FBS and 10% DMSO(v/v) Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 derivative:ATCC SCRC-1008.1 derivative:ATCC SCRC-1008.2 |
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文献和实验, we may find much more references regarding this issue. http://books.google.com/books?id=3mgPzNFjcWAC&pg=PA51&lpg=PA51&dq=gsk+tyrosine+phosphorylation+and+activity&source=bl&ots=x5ueTldpez&sig=CqRFJleXtEBn9OfelhODPAc28xs&hl=en&ei=KerNSu-XGcem8Abm-qmABA&sa=X&oi=book_result&ct
起，再在其后标明杂交的代数（如F1、F2等）。 对品系或种群的名称可使用通用的缩写名称。 例如：（C57BL/6 DBA/2）F1 = B6D2F1 （ NMRI X LAC ）F2 3 实验动物的繁殖方法 由于近交系与封闭群动物具有不同的遗传特点，因此应分别选择相应的繁殖方法。 3.1 近交系动物的繁殖方法 选择近交系动物繁殖方法的原则是保持近交系动物的同基因性及其基因纯合性。 3.1.1 引种 作为繁殖用原种的近交系动物必须遗传背景明确
2.3 杂交群 hybrids 2..3.1 定义 杂交群：由不同品系或种群之间杂交产生的后代。 2.3.2 命名 杂交群应按以下方式命名：此雌性亲代名称在前，雄性亲代名称居后，二者之间以大写英文字母"X"相连表示杂交。将以上部分用括号括起，再在其后标明杂交的代数（如F1、F2等）。 对品系或种群的名称可使用通用的缩写名称。 例如：（C57BL/6 DBA/2）F1 = B6D2F1 （ NMRI X LAC ）F2 3 实验动物的繁殖
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