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- 2025年11月22日
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- 文献和实验
- 技术资料
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 库存：
大量
- 免疫类型：
IgG2a
- 细胞形态：
淋巴样
- 运输方式：
冻存运输
- 生长状态：
悬浮生长
- ATCC Number：
HB-9282™
| Designations: | W4F.5B | ||
| Depositors: | T Cell Sciences, Inc. | ||
| Isotype: | IgG2a | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against a major framework determinant on human T cell antigen receptor | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | No other information is available. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| Doubling Time: | 24 hrs | ||
| References: | 3919: Brown MC, et al. Assay systems for detecting cell-free T cell antigen receptor related molecules and the clinical utilities of the assays. US Patent 4,845,026 dated Jul 4 1989 | ||
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文献和实验甲基化芯片背景甲基化芯片原理：https://www.jianshu.com/p/c4f758e0399d芯片主要分为 EPIC 和 450k 两种，EIPC 也就是 850k，两种探针的都是以 cg 开头的数字编号，所谓注释也就是提取这些探针的所对应的信息，例如，探针序列的 CpG 位置信息，对应的基因信息，染色体上的位置信息等等。很多包在安装的时候都会自动下载这些注释信息，并包装在一起，如果我们想要自己注释这些探针，就要考虑如何获取独立的注释信息。而所需要注释数据的，大部分都来自于两个
The STINT‐NMR Method for Studying In‐cell Protein‐Protein Interactions
in the interaction surface of the molecule rather than changes in tertiary or quaternary structure. (A ) Y371/4F‐STAM2‐Ubq interaction; (B ) phosphorylated Y371/4F‐STAM2‐Ubq interaction (YP‐Y371/4F‐STAM2). Ubiquitin ligands are indicated in each panel
7.5. 3. 10% (w/v) NaN3 . 4. Filter bottle, 0.5–1 L, 0.2-μm pore size (Corning or equivalent). 2.3. Fractionation of Wnt3A CM 2.3.1 Step 1: Blue Sepharose 1. Sample: 1–4 L Wnt3A CM as prepared in Section 2.2 . 2. Column: Blue Sepharose HP
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