![C5 [1F10]](https://custom.dxycdn.com/trademd/upload/pic/2012/03/29/1332499969.jpg!small.t.1)
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- 详细信息
- 技术资料
- ATCC Number：
HB-8669™
- 运输方式：
冻存运输
- 生长状态：
悬浮生长
- 免疫类型：
IgM
- 细胞形态：
淋巴样
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 库存：
大量
| Designations: | C5 [1F10] | ||
| Depositors: | Stanford University Board of Trustees | ||
| Isotype: | IgM | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | human (B cell); human x mouse (heteromyeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against lipid A of E. coli 0111:B4 endotoxin | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | Lymphocytes were obtained from the spleen of a patient who had been given a vaccine prepared from the J5 mutant of E. coli 0111:B4. B lymphocytes were transformed with Epstein-Barr virus and then fused with SHM-A6 mouse/human heteromyeloma cells. The antibody is specific for the lipid A of the endotoxin core of Gram negative bacteria. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%. Temperature: 37.0°C |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| Preservation: | culture medium 95%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005 recommended serum:ATCC 30-2020 |
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| References: | 22071: Kaplan HS, et al. Human monoclonal antibody to lipid A of gram negative bacteria. US Patent 5,426,046 dated Jun 20 1995 | ||
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C5 [1F10]
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