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- 2025年11月24日
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- 详细信息
- 技术资料
- 运输方式：
冻存运输
- 免疫类型：
IgG1
- 细胞形态：
淋巴样
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- ATCC Number：
CRL-1779™
- 生长状态：
悬浮生长
- 库存：
大量
| Designations: | MLH2 | ||
| Depositors: | SC Darr | ||
| Isotype: | IgG1 | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against the light harvesting chlorophyll a/b protein complex (LHC-II) of higher plants | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Tumorigenic: | Yes | ||
| Comments: | Animals were immunized with purified LHC-II. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts specifically with the 28000 dalton polypeptide of the chloroplast thylakoid. This antibody and that of MLH1 (see ATCC CRL-1766 ) may be useful in studies of the LHC-II gene family (Cab) and in studying the photoregulation of the Cab genes in chloroplast development. Tested and found negative for ectromelia virus (mousepox). |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium:
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| References: | 1317: Darr SC, et al. Monoclonal antibodies to the light-harvesting chlorophyll a/b protein complex of photosystem II. J. Cell Biol. 103: 733-740, 1986. PubMed: 3528171 | ||
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MLH2
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