![K7M2 wt [K7M2-WT]](https://custom.dxycdn.com/trademd/upload/pic/2012/03/29/1332499969.jpg!small.t.1)
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- 详细信息
- 询价记录
- 技术资料
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 细胞形态：
其他
- 细胞类型：
其他细胞类型
- 器官来源：
骨
- 库存：
大量
- 运输方式：
冻存运输
- 相关疾病：
骨肉瘤
- ATCC Number：
CRL-2836™
- 生长状态：
贴壁生长
- 品系：
BALB/c
| Designations: | K7M2 wt [K7M2-WT] | ||
| Depositors: | C Khanna | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Mus musculus | ||
| Morphology: | osteoblast |
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| Source: | Strain: BALB/c Organ: bone Disease: osteosarcoma Derived from metastatic site: lung Cell Type: osteoblast; |
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| Cellular Products: | decorin [89453 ] secreted phosphoprotein 1 (osteopontin) [89453 ] villin 2 (ezrin) [89454 ] |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Isolation: | Isolation date: 1998 (The K7M2 wt cell line was derived by intra-osseous injection of the K7 murine osteosarcoma cell line to the proximal tibia of a BALB/c mouse. The resultant primary tumor spontaneously metastasized to the lungs. A pulmonary metastasis was removed from the lung and then surgically implanted to a paraosteal tibial muscle flap of a na�ve mouse. This cycle of implantation of pulmonary metastasis to paraosteal tibial muscle flap sites was repeated, resulting in the K7M2 primary tumor. This tumor was dissociated to a single cell suspension to create the K7M2 wt cell line) | ||
| Applications: | tumor model | ||
| Tumorigenic: | Yes | ||
| Comments: | Antigen Expression: CD31 + [PubMed:1315100] Products: factor VIII [PubMed: 11315100]; integrin binding sialoprotein (BSP) [PubMed11315100]; biglyan [PubMed: 11315100]; decorrin [PubMed:1315100]; villin 2 (ezrin) [PubMed: 11325848]. Expression of bone sialoprotein, biglyan, decorrin, and osteopontin is suggestive of bone lineage cells. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
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| Subculturing: | Protocol:
Interval: Maintain cultures at a cell concentration between 5 X 10(3) and 3 X 10(5) cells/cm2 Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended Medium Renewal: Two to three times weekly |
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Doubling Time: | 31 hours | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 derivative:ATCC CRL-2837 |
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| References: | 89453: Khanna C, et al. An orthotopic model of murine osteosarcoma with clonally related variants differing in pulmonary metastatic potential. Clin. Exp. Metastasis 18: 261-271, 2000. PubMed: 11315100 89454: Khanna C, et al. Metastasis-associated differences in gene expression in a murine model of osteosarcoma. Cancer Res. 61: 3750-3759, 2001. PubMed: 11325848 89468: Khanna C, et al. The membrane-cytoskeleton linker ezrin is necessary for osteosarcoma metastasis. Nat. Med. 10: 182-186, 2004. PubMed: 14704791 |
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K7M2 wt [K7M2-WT]
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