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- 技术资料
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 细胞形态：
其他
- 细胞类型：
其他细胞类型
- 器官来源：
骨
- 库存：
大量
- 运输方式：
冻存运输
- 相关疾病：
骨肉瘤
- ATCC Number：
CRL-2836™
- 生长状态：
贴壁生长
- 品系：
BALB/c
| Designations: | K7M2 wt [K7M2-WT] | ||
| Depositors: | C Khanna | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Mus musculus | ||
| Morphology: | osteoblast |
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| Source: | Strain: BALB/c Organ: bone Disease: osteosarcoma Derived from metastatic site: lung Cell Type: osteoblast; |
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| Cellular Products: | decorin [89453 ] secreted phosphoprotein 1 (osteopontin) [89453 ] villin 2 (ezrin) [89454 ] |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Isolation: | Isolation date: 1998 (The K7M2 wt cell line was derived by intra-osseous injection of the K7 murine osteosarcoma cell line to the proximal tibia of a BALB/c mouse. The resultant primary tumor spontaneously metastasized to the lungs. A pulmonary metastasis was removed from the lung and then surgically implanted to a paraosteal tibial muscle flap of a na�ve mouse. This cycle of implantation of pulmonary metastasis to paraosteal tibial muscle flap sites was repeated, resulting in the K7M2 primary tumor. This tumor was dissociated to a single cell suspension to create the K7M2 wt cell line) | ||
| Applications: | tumor model | ||
| Tumorigenic: | Yes | ||
| Comments: | Antigen Expression: CD31 + [PubMed:1315100] Products: factor VIII [PubMed: 11315100]; integrin binding sialoprotein (BSP) [PubMed11315100]; biglyan [PubMed: 11315100]; decorrin [PubMed:1315100]; villin 2 (ezrin) [PubMed: 11325848]. Expression of bone sialoprotein, biglyan, decorrin, and osteopontin is suggestive of bone lineage cells. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
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| Subculturing: | Protocol:
Interval: Maintain cultures at a cell concentration between 5 X 10(3) and 3 X 10(5) cells/cm2 Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended Medium Renewal: Two to three times weekly |
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Doubling Time: | 31 hours | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 derivative:ATCC CRL-2837 |
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| References: | 89453: Khanna C, et al. An orthotopic model of murine osteosarcoma with clonally related variants differing in pulmonary metastatic potential. Clin. Exp. Metastasis 18: 261-271, 2000. PubMed: 11315100 89454: Khanna C, et al. Metastasis-associated differences in gene expression in a murine model of osteosarcoma. Cancer Res. 61: 3750-3759, 2001. PubMed: 11325848 89468: Khanna C, et al. The membrane-cytoskeleton linker ezrin is necessary for osteosarcoma metastasis. Nat. Med. 10: 182-186, 2004. PubMed: 14704791 |
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文献和实验相关实验
expolee 大家好，最近检测肿瘤markerA表达，qPCR和WT检测肿瘤细胞是高表达的，收集病人标本做成组织芯片后免疫组化检测则是：肿瘤组织弱表达、正常组织弱表达，怎么解释呢？尽管IHC只是定位，可弱表达的话有没有再往下做下去的意义了？欢迎大家发言，谢谢！ shylook 组织标本保存问题 IHC是否规范 是否摸过抗体使用浓度 包括二抗 一抗能能否做IHC expolee
Enzyme Kinetics assay of the WT
To assay 17 b-HSD activity in lysates, cells were harvested 48h after transfection using PBS Enzyme Free Cell Dissociation Solution ( specialty Media Inc., Lavellette, NJ), frozen on dry ice and stored at -80 C. Cell pellets were thawed
【求助】请教一下各位大虾：质粒pACYC184和pBRM-Wt的具体结构是怎样的呢，从哪可以得到，谢谢！
西点6688 请教一下各位大虾：质粒pACYC184和pBRM-Wt的具体结构是怎样的呢，谢谢！ slytjiaofei http://genome-www.stanford.edu/vectordb/vector.html 自己查查吧！！！ lastone9853 这两个质粒pACYC184和pBRM-Wt的图谱，序列等可以在网上查到，我这有这两个载体，可以快
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K7M2 wt [K7M2-WT]
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