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MiCl1 (S+L-)

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  • 2025年11月22日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 年限

      near term

    • 库存

      大量

    • 物种来源

      其他

    • 是否是肿瘤细胞

      0

    • 运输方式

      冻存运输

    • 细胞形态

      成纤维样

    • 生长状态

      贴壁生长

    • 器官来源

    • ATCC Number

      CCL-64.1™

    Designations: MiCl1 (S+L-)
    Depositors:  PT Peebles
    Biosafety Level: 1
    Shipped: frozen
    Medium & Serum: See Propagation
    Growth Properties: adherent
    Organism: Mustela vison deposited as Mustella vison
    Morphology: fibroblast

    Source: Organ: lung
    Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
    Age: near term
    Comments: Infected with Moloney murine sarcoma virus.
    Foci are formed when the cells are superinfected with mink tropic MuLV.
    Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
    Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
    Medium Renewal: Twice per week
    Remove medium, add fresh 0.25% trypsin - 0.03% EDTA solution for 1, remove trypsin and let culture sit at 37C for 5 minutes. Add fresh medium, aspirate and dispense into new flasks.
    Preservation: growth medium, 95%; DMSO, 5%
    References: 22188: Peeples PT, et al. Murine sarcoma virus defectiveness. Viral polymerase expression murine and nonmurine host cells transformed by S+L-type murine sarcoma virus. Virology 67: 344-355, 1975. PubMed: 52940

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