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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 物种来源:
其他
- 是否是肿瘤细胞:
0
- 器官来源:
淋巴结
- 生长状态:
悬浮生长
- 库存:
大量
- 细胞形态:
淋巴样
- 相关疾病:
其他疾病
- ATCC Number:
CRL-8135™
- 运输方式:
冻存运输
| Designations: | MDTC-RP19 | ||
| Depositors: | K Nazerian | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Meleagris gallopavo | ||
| Morphology: | lymphoblast |
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| Source: | Organ: lymph node Disease: Marek's disease |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | The line is free of major oncogenic avian viruses. The cells are pathogenic in chickens but fluids are not. The cells grow well at 41C. |
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| Propagation: | ATCC complete growth medium: A 1:1 mixture of Leibovitz's L-15 medium and McCoy's 5a medium with 2 mM L-glutamine, 55%;tryptose phosphate broth, 5%;chicken serum, 20%;fetal bovine serum, 20%The fetal bovine serum concentration may be reduced to 10% after the cells have been in culture for 10 to 14 days. Temperature: 41.0°C Atmosphere: air, 95%; carbon dioxide (CO2 ), 5% |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 10 exp5 viable cells/ml. Maintain cultures at cell concentrations between 5 X 10 exp5 and 2 X 10 exp6 viable cells/ml. |
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| Preservation: | Culture medium, 90%; DMSO, 10% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2007 Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008 recommended serum:ATCC 30-2020 |
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| References: | 22369: Nazerian K, et al. Establishment of B-lymphoblastoid cell lines from Marek's disease virus- induced tumors in turkeys. Int. J. Cancer 29: 63-68, 1982. PubMed: 6277804 | ||
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文献和实验clone RP23-144E24 from 1, complete sequence Length = 200656 Score = 32.2 bits (16), Expect = 42 Identities = 16/16 (100%) Strand = Plus / Plus Query: 4 aggaaagtcgcaaagc 19 |||||||||||||||| Sbjct: 11670 aggaaagtcgcaaagc 11685 >gi|56382145
LMP2B EBV EBNA1 EBV EBNA2 EBV BZLF1 EBV BRLF1 EBV BcLF1 EBV BLLF1 HLA-DR alpha TNF-alpha supar-domain1 VEGF165 RP2 RP3 exon1 RP3 exon2 RP3 exon3~6 RP3 exon7~10 RP3 exon11~14 RP3 exon15~19 DYPD ICAM-I VCAM 1 BCL2L1 survivin
| Mus musculus chromosome 3, clon... 40 0.20 gi|41393315|gb|AC105297.35| Mus musculus clone rp23-14k19 m... 40 0.20 gi|40352726|gb|BC064681.1| Mus musculus similar to glyceral... 40 0.20 gi|41054967|ref|NM_199472.1| Mus musculus similar to glycer
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