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阿拉丁试剂，充足现货，稳定批间差Cytoscape 教程来了！快速实现顶刊同款通路网络图五大应用案例：Mustang Q 膜层析应用全解析1 个小工具，一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 文献和实验
- 技术资料
- 运输方式：
冻存运输
- 物种来源：
其他
- 是否是肿瘤细胞：
0
- 库存：
大量
- 相关疾病：
正常
- 器官来源：
肾脏
- ATCC Number：
CRL-1922™
- 细胞形态：
成纤维样
- 生长状态：
贴壁生长
| Designations: | NZP-36 | ||
| Depositors: | L Munson | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Equus burchelli | ||
| Morphology: | fibroblast |
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| Source: | Organ: kidney Disease: normal |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | The cells can be propagated for at least 10 population doublings beyond frozen reference seed stock. | ||
| Propagation: | ATCC complete growth medium: Dulbecco's modified Eagle's medium, 45%; Ham's F12 medium, 45%; fetal bovine serum, 10%; plus the following additives: 10 ng/ml epidermal growth factor; 0.005 mg/ml insulin; 5 ng/ml selenium; 0.005 mg/ml transferrin |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: Every 2 to 3 days Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Allow the flask to sit at room temperature, or incubate at 37C, until the cells detach (about 5 to 10 minutes). Add fresh medium, aspirate and dispense into new flasks. |
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文献和实验CD36 分子 CD36 常用单克隆抗体或代号： 5F1,ESIVC7,OKM5 主要表达细胞： Pt,M,Mac, (B) [Pt] 分子质量(kDa)和结构： gp88,血小板GPⅣ 功 能： 结合ECM(CA、Thr),血小板粘附 CD36 Aka platelet GPIV or GPIIIb; thrombospondin receptor Scavenger
36 Large-Scale Extraction of Proteins
Purification of proteins using aqueous two-phase systems (ATPS) has been successfully carried out on large scale for more than a decade (1 –17 ). One of the major advantages of ATPS is that the scaling up is possible through the use
人可溶性CD36 分子 ( sCD36 )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 sCD36 单抗包被于酶标板上，标准品和样品中的 sCD36与单抗结合，加入生物素化的抗人 sCD36 ，形成免疫复合物连接在板上，辣根过氧化物酶标记的 Streptavidin 与生物素结合，加入底物工作液显蓝
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