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- 品系：
BALB/c
- 库存：
大量
- 免疫类型：
IgM (surface)
- 细胞形态：
淋巴样
- 器官来源：
胸腺
- 生长状态：
悬浮生长
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 运输方式：
冻存运输
- 相关疾病：
淋巴瘤
- ATCC Number：
TIB-54™
| Designations: | WEHI 22.1 | ||
| Depositors: | AW Harris | ||
| Isotype: | IgM (surface) | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus | ||
| Morphology: | lymphoblast |
||
| Source: | Organ: thymus Strain: BALB/c Disease: lymphoma |
||
| Cellular Products: | immunoglobulin; monoclonal antibody | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Antigen Expression: | Thy-1.2 | ||
| Comments: | The line was derived from a thymoma induced by X-irradiation. The cells have characteristics of both T and B cells and form rosettes with sheep erythrocytes. They are sensitive to 100 nM cortisol. Tested and found negative for ectromelia virus (mousepox). |
||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
||
| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
||
| References: | 1134: Harris AW, et al. Differentiated functions expressed by cultured mouse lymphoma cells. II. theta Antigen, surface immunoglobulin and a receptor for antibody in cells of a thymoma cell line. J. Immunol. 110: 431-438, 1973. PubMed: 4630643 | ||
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