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- 详细信息
- 文献和实验
- 技术资料
- 生长状态：
贴壁生长
- ATCC Number：
CRL-2773™
- 库存：
大量
- 物种来源：
其他
- 是否是肿瘤细胞：
0
- 细胞形态：
上皮样
- 细胞类型：
上皮细胞
- 器官来源：
胚胎
- 运输方式：
冻存运输
- 年限：
24 hours post fertilization embryo
| Designations: | WBE | ||
| Depositors: | M Westerman | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Morone chrysops | ||
| Morphology: | epithelial |
||
| Source: | Organ: embryo Cell Type: epithelial |
||
| Cellular Products: | keratin [90251 ] | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Isolation: | San Diego California, Isolation date: February 22, 2001 |
||
| Cytogenetic Analysis: | Aneuploid. Range = 44 to 49. The chromosme morphology was normal for bass with all telocentric and acrocentric chromosomes. No dicentric or ring chromosomes were observed. [90251 ] | ||
| Age: | 24 hours post fertilization embryo | ||
| Gender: | unknown | ||
| Comments: | These cells are useful for the study of host-pathogen interactions. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 31.0°C |
||
| Subculturing: | Protocol:
Subcultivation Ratio: 1:4 to 1:6 weekly Medium Renewal: Every 2 to 3 days |
||
| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 |
||
| References: | 90251: Shimizu C, et al. Characterization of a white bass (Morone chrysops) embryonic cell line with epithelial features. In Vitro Cell. Dev. Biol. Anim. 39: 29-35, 2003. PubMed: 12892524 | ||
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+THulV/kl7R/6hmn21HydXzSqafYCXNmu59isV5NEtRfEWR3JiGCXK+VLKeV+b785b+240443s44a64WPZlpS5Y86eT7s//5s7b06RftM1pJcP2q9TZ37pnyczxPv3PtvPPOs507d3pLup9jbLVjiAjsIwj8R+7ofaRqUcyIQEQgIhARiAi8FQj89qGVyXq/PaBJVtpqRv6Vh8gX8Uat1rfElklz+9nPfdoee/wBe+KpX2q56PPtxeUvSwO80
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