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- 文献和实验
- 技术资料
- 运输方式：
冻存运输
- 免疫类型：
IgM; lambda light chain
- 细胞形态：
淋巴样
- 相关疾病：
淋巴瘤
- ATCC Number：
TIB-221™
- 品系：
B10.H-2aH-4bp/Wts(2a4b)
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 细胞类型：
B淋巴细胞
- 库存：
大量
- 生长状态：
悬浮生长
| Designations: | CH1 | ||
| Depositors: | G Haughton | ||
| Isotype: | IgM; lambda light chain | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus | ||
| Morphology: | lymphoblast |
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| Source: | Strain: B10.H-2aH-4bp/Wts(2a4b) Disease: lymphoma Cell Type: B lymphocyte; |
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| Cellular Products: | immunoglobulin (surface); against E. coli | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | transfection host (Roche Transfection Reagents ) | ||
| Antigen Expression: | I-Ak; I-Ek | ||
| Comments: | This line was established in tissue culture from the CH1 B cell lymphoma. The cells produce, but do not secrete, immunoglobulin. Tested and found negative for ectromelia virus (mousepox). |
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| Propagation: | ATCC complete growth medium: RPMI 1640 medium with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10% |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 x 10 exp4 cells/ml and maintain between 3 x 10 exp5 and 8 x 10 exp5 cells/ml. |
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| References: | 22178: Lanier LL, et al. Transplantable B-cell lymphomas in B10. H-2aH-4bp/Wts mice. Immunogenetics 16: 367-371, 1982. PubMed: 6983495 22235: Lanier LL, et al. Novel type of murine B-cell lymphoma. Nature 271: 554-555, 1978. PubMed: 304532 22283: . . J. Immunol. 121: 2353, 1978. |
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文献和实验Use of HMS Genetics Confocal Microscope
• Reset the resolution to 1024, the number of sections to 4-8, and the accumulations or passes per section to 8-16. • Set the view to [gallery], [tiled], [Ch1], [Ch2]. • Press the [series] icon. saving images • To save images
Ig分子的每一条肽链，都由链内二硫键将相邻的二级结构单元折叠成球形局部性区域（图2-2）；每个球形区约由110个氨基酸残基组成，不同Ig分子对应球形区的氨基酸残基顺序具有高度的相似性，因此称为同源区（domain）。IgG、IgA和IgD分子共有12个同源区，其中Lκ或Lλ各2个：VL和CL；H链各4个：VH、CH1、CH2和CH3.IgM和IgE分子各有14个同源区，因为其H链上多一个CH4.虽然H链和C区的不同区域彼此同源，并且与L链的CL同源，但它们与V区的氨基酸排列顺序极少相同
Introduction to Heavy Chain Antibodies and Derived Nanobodies
and in the case of the heavy chain antibodies in camelids the CH1 domain is also missing. Consequently a camelid heavy chain antibody associates with its cognate antigen via a single domain, the variable heavy chain domain of a heavy chain antibody or VHH
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