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- 文献和实验
- 技术资料
- 运输方式：
冻存运输
- 物种来源：
仓鼠
- 是否是肿瘤细胞：
0
- 库存：
大量
- 器官来源：
卵巢
- 细胞形态：
上皮样
- 生长状态：
贴壁生长
- ATCC Number：
CRL-2348™
| Designations: | xrs5 | ||
| Depositors: | G Chu | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Cricetulus griseus | ||
| Morphology: | epithelial |
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| Source: | Organ: ovary | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Gender: | female | ||
| Comments: | xrs5 is a X-ray sensitive Chinese Hamster Ovary mutant cell line which was derived from CHO-K1 cells by treating the cells with ethyl methanesulphonate and subsequent growth in agar. These cells belong to X-ray complementation group 5 and are mutant in the p86 subunit of the Ku autoantigen. Ku has been shown to be involved in DNA double-strand break repair and V(D)J recombination. The cells will revert to wild type on treatment with azacytidine. |
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| Propagation: | ATCC complete growth medium: Alpha minimum essential medium without ribonucleosides and deoxyribonucleosides with 2 mM L-glutamine, 90%; fetal bovine serum, 10% Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended Medium Renewal: Every 2 to 3 days Remove medium, rinse quickly with 0.25% trypsin, 0.03% EDTA solution, add 1-2 ml additional trypsin solution and allow flasks to set at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new flasks. |
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| Preservation: | culture medium 95%; DMSO, 5% | ||
| References: | 37303: Jeggo PA, Kemp LM. X-ray-sensitive mutants of Chinese hamster ovary cell line. Isolation and cross-sensitivity to other DNA-damaging agents. Mutat. Res. 112: 313-327, 1983. PubMed: 6197643 37304: Rathmell WK, Chu G. Involvement of the Ku autoantigen in the cellular response to DNA double-strand breaks. Proc. Natl. Acad. Sci. USA 91: 7623-7627, 1994. PubMed: 8052631 37305: Rathmell WK, Chu G. A DNA end-binding factor involved in double-strand break repair and V(D)J recombination. Mol. Cell. Biol. 14: 4741-4748, 1994. PubMed: 7516471 |
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文献和实验10 N NaOH method: For 100 ml vanadyl complex, Make 10 ml 2 M vanadyl sulfate (0.366 g/ml) To make 80 ml H2O in a 250 ml flask, add 1.34 g adenosine, 1.45 g guanosine, 1.25 g uridine, and 1.25 g cytidine = 5 mM XRs Boil in H2
Imaging the Quansys Q-Plex multiplex ELISA array using the Bio Rad Chemi Doc XRS imaging system. " Bio-rad凝胶成像系统的使用 "
In Vitro Monitoring of Base Excision Repair in Saccharomyces cerevisiae
of base damage and of individual mechanistic steps within BER. We used these protocols to investigate a new role of the DNA double strand break repair protein XRS1 in BER (1).
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