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- 相关疾病：
正常
- ATCC Number：
CRL-2936™
- 细胞形态：
上皮样
- 物种来源：
狗
- 是否是肿瘤细胞：
0
- 库存：
大量
- 年限：
adult
- 运输方式：
冻存运输
- 器官来源：
其他
- 生长状态：
贴壁生长
| Designations: | MDCK.2 | ||
| Depositors: | Y. Reid, E. Cedrone and E-Eckard-Amar, ATCC | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Canis familiaris | ||
| Morphology: | epithelial-like |
||
| Source: | Organ: kidney; distal tubule Disease: normal |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Isolation: | Isolation date: Jan 2007 | ||
| Virus Susceptibility: | Influenza A virus Influenza B virus |
||
| Antigen Expression: | E-cadherin (epithelial cell adhesion molecule), expressed Zona Occludens (ZO-1) (tight junction protein), expressed fibroblast-specific protein (FSP), not expressed cytokeratin (CK1, 4, 5, 6, 8, 10, 13, 18, 19),expressed sialic receptors: alpha 2,3-galactose (avian)and alpha 2,6-galactose (human); expressed |
||
| Cytogenetic Analysis: | hyperdiploid canine cell line with a modal chromosome number of 91 with low polyploidy rate. Several unidentifiable marker chromosomes were present in most of the cells examined. | ||
| Age: | adult | ||
| Comments: | Cell line was derived by cloning (limited dilution) the parental cell line MDCK (CCL-34. This cell line is susceptible to a wide range of influenza virus and is sensitive to epsilon toxin of C. perfringens . | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
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| Subculturing: | Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
|
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| Preservation: | Freeze medium: Complete growth medium, 95%; DMSO, 5% liquid nitrogen vapor phase |
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| Doubling Time: | approximately 22 hours | ||
| Related Products: | Recommended medium (without the additional serum described under ATCC Medium): ATCC 30-2003 Recommended serum: ATCC 30-2020 0.25% (w/v) Trypsin - 0.53mM EDTA in Hank's BSS (w/o Ca++, Mg ++): ATCC 30-2101 Phosphate-buffered saline: ATCC 30-2200 Cell culture tested DMSO: ATCC 4-X Erythrosin B vital stain solution: ATCC 30-2404 parental cell line - CCL-34 derived from same individual - CRL-2935 |
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| References: | 16173459: Cedrone E, et al. Tissue-culture adapted Influenza virus strains. ATCC Connection volume 29 (2): 4-5 and 15, 2009. | ||
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MDCK.2
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