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100 µg
蛋白名称:CD166/ALCAM蛋白, CD166/ALCAM protein
蛋白构建:A DNA sequence encoding the human ALCAM (NP_001618.2) (Met1-Ala526) was expressed with the Fc region of human IgG1 at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 90 % as determined by SDS-PAGE.
蛋白活性:0
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Trp 28
蛋白分子量:The recombinant human ALCAM consists of 737 amino acids and predicts a molecular mass of 82.7 kDa.
蛋白NP号:NP_001618.2
蛋白氨基酸序列:Met1-Ala526
蛋白标签:C-human IgG1-Fc
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Determination of Epitopes by Mass Spectrometry
located on the gp41 protein of the human immunodeficiency virus recognized by the monoclonal antibody 2F5. In this approach we coupled the antigen SOSgp140 to the antibody 2F5, which was covalently linked to an Fc-specific antibody immobilized on cyanogen
L? Protein A—只与 Fc 区域结合 Protein G—结合 Fc 区域和部分轻链 Protein A/G—重组蛋白,兼具 Protein A 和 Protein G 的结合位点 Protein L—结合仅限于那些含有 κ 轻链的抗体 省事且通用可选择 Protein A/G,也可根据结合力表格进行选择,详见资源信息部分 3、基质—琼脂糖还是磁珠? 琼脂糖 磁珠 优点 简单易用,无需辅助设备 操作简单 孔径大,直径大(50-150
Capture and Qualitative Analysis of the Activated Fc Receptor Complex from Live Cells
the isolation of activated Fc receptor complexes from RAW 264.7 macrophages using live?cell affinity receptor chromatography (LARC). The Fc receptor complex is activated and captured by IgG?coated microbeads on the surface of live macrophages. After the cells
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