重组人 KIRREL1 / NEPH1 蛋白 (His标签)

规格/价格
100 µg：3870
1 mg：25190

KIRREL Protein, Human, Recombinant (His Tag): 产品信息

• 纯度：> 95 % as determined by SDS-PAGE.
• 内毒素：< 1.0 EU per μg protein as determined by the LAL method.
• 生物活性：Measured by the ability of the immobilized protein to support the adhesion of MS1 mouse pancreatic islet endothelial cells.
• When cells are added to KIRREL coated plates (15 μg/mL, 100 μL/well), > 20% will adhere specifically after 90 minutes at 37 ℃.
• 蛋白构建：A DNA sequence encoding the human KIRREL (NP_060710.3) (Met1-Ala499) was expressed with a polyhistidine tag at the C-terminus.
• 表达宿主：HEK293 Cells
• 种属：Human
• 预测 N 端：Gln 17
• 分子量：The recombinant human KIRREL consists 488 amino acids and predicts a molecular mass of 53.5 kDa.
• 缓冲液

1. Lyophilized from sterile PBS, pH 7.4.
2. Please contact us for any concerns or special requirements.
3. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
4. Please refer to the specific buffer information in the hard copy of CoA.

• 运输方式

1. In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
2. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

• 稳定性 & 储存条件

1. Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
2. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

• 复溶：A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

KIRREL Protein, Human, Recombinant (His Tag): 别称
NEPH1 Protein, Human

KIRREL 背景信息
NEPH1 (KIRREL1) belongs to a family of three closely related transmembrane proteins of the Ig superfamily with a structure similar to that of nephrin. All three Neph proteins share a conserved podocin-binding motif; mutation of a centrally located tyrosine residue dramatically lowers the affinity of Neph1 for podocin. Neph1 triggers AP-1 activation similarly to nephrin but requires the presence of Tec family kinases for efficient transactivation. Neph1 consists of a signal peptide, five Ig-like C2-type domains with the middle domain overlapping with a PKD-like domain, an RGD sequence, a transmembrane domain, and a cytoplasmic tail, which is expressed in slit diaphragm domains of podocytes and vertebrate and invertebrate nervous systems. Neph1 is abundantly expressed in the kidney, specifically expressed in podocytes of kidney glomeruli, and plays a significant role in the normal development and function of the glomerular permeability. Neph1 interacts with nephrin in vitro and in vivo, and able to stimulate transcriptional activation in a model system, such as the activation of the transcription factor AP-1 via the stimulation of a MAPK module. Neph1 is crucial for the integrity of the slit diaphragm, as Neph1 gene knockout mice result in effacement of glomerular podocytes, heavy proteinuria, and early postnatal death.

全称
kin of IRRE like (Drosophila)