At 37℃ activity is 75%from maximum.To obtain 100%activity,BSA should be added to the 1 x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation.
技术规格
Unit definition
One unit of the enzyme is the amount required to hydrolyze 1 μg of pBR322 DNA in 1hourat 55℃ in a total reaction volume of 50μl.
Recognition sites
↑AATT
来源
E.coli strain that carries the cloned Sse9 I gene from Sporosarcina species
Assayed on
pBR322 DNA
Optimal buffer
B?(10mMTris-HCl(pH 7.6 at 25℃);10mMMgCl2;1 mM DTT.)+?BSA
Optimal temperature
55℃
Storage conditions
10mMTis-HCl(pH 7.5);50 mM NaCl;0.1 mM EDTA;1 mM DTT;200 μg/ml BSA;50%glycerol.Store at -20℃.
Ligations
After 5-fold overdigestion with enzyme more than 95%of the DNA fragments can be ligated and recut.
Non-specific hydrolisis
No nonspecific activity was detected after incubation of 1 μg of DNA with 5 u.a.of enzyme for 16hoursat 55℃.
Methlation effect
Methylation sensitivity not tested
Thermal inactivation
enzyme is inactivated by incubation at 65℃ for 20 mins.