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- 菁良基因GeneWell
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文献和实验1 A260 unit of duplex DNA = 50 ug 1 A260 unit of single-stranded DNA = 37 ug 1 A260 unit of single-stranded RNA = 50 ug 1 kb of DNA = 333 amino acids of coding capacity = 37,000 daltons Densities (50% GC
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
, put directly on slides w/ stain 4) inc o/n 37C in humidity chamber 5) asp, inc 10''in FAA: for 200ml (4C) 10ml formaldehyde 10ml HAc 75ml EtOH H2O > vol 6) inc 2'' 50% EtOH 7) inc 2'' 100% EtOH 8) inc 1'' H2O GUS
Standard neutral agarose electrophoresis
8. 1 x TAE buffer - 40mM Tris-acetate, 2mM EDTA, pH 8. 10mg/ml ethidium bromide stock solution 10 x Type III loading buffer (50% glycerol, 0.5% bromophenol blue, 0.5% xylene cyanol, this solution should be stored at 4°C) N.B: Extreme caution
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