SpectraView光谱成像分析系统

Feature

Details / Benefits

Spectral range: 400-1000nm

Full coverage of the visual range (400-700nm) as well as NIR till 1000nm in a single system, single capture. Nuance does not capture the deep blue region (below 420nm) which is part of the visual range.

Spectral resolution of 6nm at 400nm

Nuance of CRI has 20nm or 40nm per channel (depend on system ordered). This is more than three times better than Nuance

User defined spectral resolution.

Optimized spectral details and signal quality

Polarized insensitive

Nuance from CRI is based on a LCTF that is sensitive to polarization, and blocks 50% of regular light

High transmission efficiency

Sensitive and derive good SNR even with faint fluorescence samples. Nuance has only 4% transmission at 420 blue (2% for non-polarized light)

full spectral range is used for Live view

Focus and image view represents full image data. Also, the intensity of the entire spectrum enables faster and more relevant focus.

(Nuance focus must be done at a specific narrow filter that does not represents the entire image and transfers limited light intensity)

16 bit spectral image layers

Higher accuracy in quantitative image representation

Dual mode system to capture both Imaging and Hyper-spectral Imaging

Direct light (all colors, non-filtered) image capture can be used as a reference image. In addition, filter based applications exists using the “direct imaging mode”.

Exposure time from 1mSec-10sec

Large exposure per frame. Enables capture strong and faintest signals.

Feature

Details / Benefits

Enables all imaging modes:Transmittance, Fluorescence,Reflection

Supports both brightfield and fluorescent applications of all types

Graphical representation of spectra:

• Original spectra
• Transmittance
• Absorption
• Optical Density
• Background subtracted

Informative spectral display to show relevant data and be valid for publication as normalized spectra.

Spectral un-mixing in brightfield as well as in fluorescence

Extracting quantitative per pixel information on molecular/stain content

Reveals “unexpected”cells/objects within the image

Dedicated image to show elements that have different spectrum than expected. Critical for various research applications.

Auto cell segmentation to identify cells and their nuclei. Performed also on spectral un-mixed images

Unique segmentation power is achieved by performing it on single un- mixed dye. E.g.: Hx (brightfield) or DAPI (fluorescence) can be used to identify the nuclei without the disturbance or overlapping dyes.

Advanced cell classification based on morphological and spectral data simultaneously.

Unique capability. Enables to classify cells to multiple pre-defined classes based on their shape and spectral characteristics (like IHC content).

Statistics table summarizing how many cells of each type were found

Immediate score of cells, for multiplex IHC staining and more

Background removal (uniform or non-uniform!)

Visualize the image without un-needed background effect

Multiple mathematical operations

between cubes, images and spectra.

Enables endless needs, including accurate normalization of spectra within the scene, cover for non-uniformities in microscope illuminations, background manipulation and more.

Load multiple cases/images simultaneously

Enables fast comparison between different regions, samples, preparations

Endless display options to show extracted single un-mixed layers, any subset of layers, classified cells, cell contours, spectral integration over specific range, spectral graphs and more.

Enables visualize the data in a simple and informative way. Paper-ready images and spectral graphs.