NF-κB p65 单克隆抗体 66535-1-Ig产品图
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NF-κB p65 单克隆抗体 66535-1-Ig

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  • ¥1350 - 3000
  • Proteintech已认证
  • 66535-1-Ig
  • 2026年05月21日
  • WB, IHC, ELISA
  • Mouse
  • Human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原:

      Recombinant human p65; RELA protein

    • 亚型:

      IgG1

    • 保存条件:

      Store at -20°C. Stable for one year after shipment.

    • 克隆性:

      Monoclonal

    • 标记物:

      Unconjugated

    • 适应物种:

      Human

    • 保质期:

      详见产品包装

    • 库存:

      充足

    • 宿主:

      Mouse

    • 应用范围:

      WB, IHC, ELISA

    • 靶点:

      NF-κB p65

    • 抗体英文名:

      NF-κB p65 Monoclonal antibody

    • 抗体名:

      NF-κB p65 Monoclonal antibody

    • 规格:

      50ul/100ul/150ul

    规格:50ul产品价格:¥1350.0
    规格:100ul产品价格:¥2200.0
    规格:150ul产品价格:¥3000.0

    经过测试的应用

    Positive WB detected inHeLa cells, HEK-293 cells, MOLT-4 cells, Jurkat cells, Raji cells
    Positive IHC detected inhuman breast cancer tissue
    Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
    Planning an IHC experiment? We recommend our IHCeasy NF-κB p65/RELA Ready-To-Use IHC Kit. NF-κB p65/RELA primary antibody included.
    Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

    推荐稀释比

    应用推荐稀释比
    Western Blot (WB)WB : 1:1000-1:4000
    Immunohistochemistry (IHC)IHC : 1:150-1:600
    It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
    Sample-dependent, Check data in validation data gallery.

    产品信息

    66535-1-Ig targets NF-κB p65 in WB, IHC, IF, CoIP, ChIP, RIP, ELISA applications and shows reactivity with Human samples.

    经测试应用 WB, IHC, ELISA
    文献引用应用WB, IHC, IF, CoIP, ChIP, RIP
    经测试反应性 Human
    文献引用反应性human, pig, chicken, bovine, hamster
    免疫原

    CatNo: Ag1199

    Product name: Recombinant human p65; RELA protein

    Source: e coli.-derived, PGEX-4T

    Tag: GST

    Domain: 1-220 aa of BC011603

    Sequence: MDELFPLIFPAEPAQASGPYVEIIEQPKQRGMRFRYKCEGRSAGSIPGERSTDTTKTHPTIKINGYTGPGTVRISLVTKDPPHRPHPHELVGKDCRDGFYEAELCPDRCIHSFQNLGIQCVKKRDLEQAISQRIQTNNNPFQVPIEEQRGDYDLNAVRLCFQVTVRDPSGRPLRLPPVLSHPIFDNRAPNTAELKICRVNRNSGSCLGGDEIFLLCDKVQ

    宿主/亚型 Mouse / IgG1
    抗体类别 Monoclonal
    产品类型 Antibody
    全称 v-rel reticuloendotheliosis viral oncogene homolog A (avian)
    别名 NF-kB, NFKB3, NF-κB, NF-κB p65, NF-κB3, p65, RELA, Transcription factor p65
    计算分子量 65 kDa
    观测分子量 65 kDa
    GenBank蛋白编号BC011603
    基因名称 NF-κB p65
    Gene ID (NCBI) 5970
    RRIDAB_2881898
    偶联类型 Unconjugated
    形式Liquid
    纯化方式Protein G purification
    UNIPROT IDQ04206
    储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
    储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

    背景介绍

    Nuclear factor k B (NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-Kb family in mammalian cells include the proto-oncogene c-Rel,p50/p105 (NFkB1), p65 (RelA), p52/p100 (NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-Kb. The p65 subunit is a major component of NF-Kb complexes and is responsible for trans-activation. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. It associates with chromatin at the NF-kappa-B promoter region via association with DDX1.

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    图标文献和实验
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    相关实验
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    NF-κB p65 单克隆抗体 66535-1-Ig
    ¥1350 - 3000