- ￥1350 - 3000
- Proteintech
- 66264-1-Ig
- 2025年10月13日
- WB, IHC, IF/ICC, FC (Intra), ELISA
- Mouse
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human Cytochrome c protein
- 亚型：
IgG2a
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Mouse
- 应用范围：
WB, IHC, IF/ICC, FC (Intra), ELISA
- 靶点：
Cytochrome c
- 抗体英文名：
Cytochrome c Monoclonal antibody
- 抗体名：
Cytochrome c Monoclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | HeLa cells, human heart tissue, human skeletal muscle tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue, HEK-293 cells, HepG2 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, ROS1728 cells, RAW 264.7 cells |
| Positive IHC detected in | human liver cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:5000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66264-1-Ig targets Cytochrome c in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), ELISA |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, canine |
| 免疫原 |
CatNo: Ag24349 Product name: Recombinant human Cytochrome c protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-105 aa of BC009578 Sequence: MGDVEKGKKIFIMKCSQCHTVEKGGKHKTGPNLHGLFGRKTGQAPGYSYTAANKNKGIIWGEDTLMEYLENPKKYIPGTKMIFVGIKKKEERADLIAYLKKATNE |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | cytochrome c, somatic |
| 别名 | CYCS, CYC, 2D8D11 |
| 计算分子量 | 12 kDa |
| 观测分子量 | 12-15 kDa |
| GenBank蛋白编号 | BC009578 |
| 基因名称 | Cytochrome c |
| Gene ID (NCBI) | 54205 |
| RRID | AB_2716798 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P99999 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. Upon apoptotic stimulation, cytochrome c can be released from mitochondria into cytoplasm, resulting in caspase-3 activation and apoptosis. Measurement of cytochrome c release from the mitochondria is useful for detection of the onset of apoptosis in cells. In addition, cytochrome c can also leave cells and be detectable in extra-cellular medium of apoptotic cells and serum of cancer patients. The level of serum cytochrome c may serve as a prognostic maker during cancer therapy.
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文献和实验| Species | Application | Title |
|---|---|---|
| human,mouse | WB | Hepatology Hepatic stimulator substance resists hepatic ischemia/reperfusion injury by regulating Drp1 translocation and activation.Authors - Chao Zhang |
| human | Cell Death Differ ZNF451 collaborates with RNF8 to regulate RNF168 localization and amplify ubiquitination signaling to promote DNA damage repair and regulate radiosensitivityAuthors - Feng Xu | |
| human | IF | Nat Commun Endonuclease G promotes autophagy by suppressing mTOR signaling and activating the DNA damage response.Authors - Wenjun Wang |
| human | WB | Redox Biol Mecheliolide elicits ROS-mediated ERS driven immunogenic cell death in hepatocellular carcinoma.Authors - Zhongren Xu |
| human | WB | ACS Appl Mater Interfaces Extracellular Matrix-Mimicking Hydrogel with Angiogenic and Immunomodulatory Properties Accelerates Healing of Diabetic Wounds by Promoting AutophagyAuthors - Yujie Pan |
| human | IF | J. Pineal Res. Human transporters, PEPT1/2, facilitate melatonin transportation into mitochondria of cancer cells: an implication of the therapeutic potential.Authors - Xiaokui Huo |
广义的细胞色素 c是含有血红素 c的细胞色素之总称。通常是指主要存在于高等动植物、酵母、霉菌等线粒体中的细胞色素 c，起着传递电子的作用。为分子量约 1.3万的碱性蛋白质，还原型的吸收带为 550、 520、 415毫微米，氧化型为 407毫微米。α 因此很早便被研究，并获得结晶。不仅一级结构而且立体结构也已清楚（图 1）。其结构基本上是生物界共有的。由细胞色素 c氧化酶〔细胞色素（ a＋ a3 ）复合体〕氧化。在呼吸链中从细胞色素 c开始接受电子，各种还原酶都已清楚
Cytochrome c Stabilization and Immobilization in Aerogels
structurally intact within the gels during the necessary supercritical fluid processing. However, the heme protein cytochrome c (cyt. c ) forms self-organized superstructures around gold (or silver) nanoparticles in buffer that can be encapsulated within silica
Assessing Cytochrome-c Release from Mitochondria
In recent years substantial evidence has accumulated demonstrating a central regulatory role for mitochondria in cell death (1 ). More recently, mechanisms of cell death, through apoptosis, have been elucidated
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