- ￥1350 - 3000
- Proteintech
- 19987-1-AP
- 2025年10月13日
- WB, IHC, IF/ICC, IP, ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
None
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, IP, ELISA
- 靶点：
LDHA-Specific
- 抗体英文名：
LDHA-Specific Polyclonal antibody
- 抗体名：
LDHA-Specific Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | HEK-293 cells, A431 cells, HeLa cells, MDA-MB-231 cells, mouse testis tissue, rat kidney tissue, Jurkat cells, NIH/3T3 cells, MCF-7 cells |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human heart tissue, human skeletal muscle tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19987-1-AP targets LDHA-Specific in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, RIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, rabbit, chicken, goat |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | lactate dehydrogenase A |
| 别名 | LDHA, Cell proliferation-inducing gene 19 protein, EC:1.1.1.27, lactate dehydrogenase A, LDH A |
| 计算分子量 | 37 kDa |
| 观测分子量 | 32-37 kDa |
| GenBank蛋白编号 | NM_005566 |
| 基因名称 | LDHA |
| Gene ID (NCBI) | 3939 |
| RRID | AB_10646429 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P00338 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Lactate dehydrogenase (LDH) is composed of A subunits predominate in skeletal muscle and B subunits are abundantly produced in brain and heart. The LDHA (lactate dehydrogenase A) and COPB1 (coatomer protein complex, subunit beta 1)genes, are involved in energy metabolism and protein transport processes. Both genes might play important roles in muscle development. It has some isoforms with the molecular mass of 27-40 kDa and can form a homotetramer(PMID:11276087). This antibody is specific to LDHA and has no cross reaction to LDHB and LDHC.
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The electrophoretic mobility shift assay (EMSA) is a sensitive relatively straightforward methodology used to detect sequence-specific DNA–protein interactions. It is the fundamental procedure of several variants that allow qualitative
element known as TRE (TPA Responsive Element) is used as an in vitro assay for AP-1 activity in the nucleus of skin cells. This activity is detected by electrophoretic mobility shift assay (EMSA) which is based on the ability of specific proteins to bind
Blocking of unwanted non-specific staining
that may affect your staining. Blocking of endogenous Alkaline Phosphatase is done by exploiting the differential sensitivity of calf intestinal AP, used as a reporter, and leukocyte AP. This latter is inhibited, while the former is not by Levamisole 1 mM
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