- ￥1350 - 3000
- Proteintech
- 17952-1-AP
- 2025年10月13日
- WB, IHC, IF/ICC, IP, ELISA
- Rabbit
- human, mouse, rat
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- 询价记录
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human PD-L1/CD274 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, IP, ELISA
- 靶点：
PD-L1/CD274
- 抗体英文名：
PD-L1/CD274 Polyclonal antibody
- 抗体名：
PD-L1/CD274 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | human placenta tissue, A375 cells, IFN gamma treated A549 cells, HepG2 cells, K-562 cells, mouse heart tissue, mouse skeletal muscle tissue, rat kidney tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human tonsillitis tissue, human stomach cancer tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17952-1-AP targets PD-L1/CD274 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, ChIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag12432 Product name: Recombinant human PD-L1/CD274 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 25-241 aa of BC074984 Sequence: KDLYVVEYGSNMTIECKFPVEKQLDLAALIVYWEMEDKNIIQFVHGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQVLSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAELVIPELPLAHPPNERTHL |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | CD274 molecule |
| 别名 | CD274, PD-L1, B7 H1, B7-H1, hPD-L1 |
| 计算分子量 | 290 aa, 33 kDa |
| 观测分子量 | 45-56 kDa, 65-70 kDa |
| GenBank蛋白编号 | BC074984 |
| 基因名称 | PD-L1 |
| Gene ID (NCBI) | 29126 |
| RRID | AB_10597552 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NZQ7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PD-L1, also known as CD274 or B7H1, stands for programmed cell death ligand 1. It is a type I transmembrane protein that is thought to repress immune responses by binding to its receptor (PD1), thus inhibiting T-cell activation, proliferation, and cytokine production. It contains V-like and C-like immunoglobulin domains. PD-L1 expression is regulated by various cytokines, such as TNF-α or LPS (ISSN: 1848-7718). Increased expression of this protein in certain types of cancers, e.g., renal cell carcinoma or colon cancer, correlates with poor prognosis.
What is the molecular weight of PD-L1?
Depending on the isoform, the calculated molecular weight of the protein varies between 20 and 33 kDa (176-290 aa).
What are the isoforms of PD-L1?
According to NCBI, three different isoforms have been identified. There are significant differences in the untranslated and protein coding regions.
What is the subcellular localization and tissue specificity of PD-L1?
It is predicted to localize in the plasma membrane of various cell types, with a particularly high expression in placental trophoblast and subsets of immune cells. High levels of PD-L1 protein have also been detected in lung and colon tissues.
What is the function of PD-L1 in immune responses?
PD-L1 is critical for the induction and maintenance of immune self-tolerance during infection or inflammation in normal tissues. The interaction of PD-L1 and its receptors is responsible for preventing auto-immune phenotypes and balancing the overall immune response in situations such as pregnancy or tissue allografts. The interaction between PD-L1 and PD-1 or B7.1 starts an inhibitory signaling cascade, which results in the decreased proliferation of antigen-specific T-cells and increased survival of regulatory T-cells (PMID: 15240681).
How can PD-L1's implication in cancer be used as a drug target?
In certain tumors, high expression of PD-L1 serves as a stop-sign to inhibit the recognition of cancer cells by T-cells (PMID: 23087408). The interaction between PD-L1 and its receptors (PD1 and B7.1) is a mechanism for the tumor to evade the host immune response (PMID: 29357948). Several mAbs have been developed to target that interaction and thus prevent the inactivation of cytotoxic T-cells by the tumor (PMIDs: 23890059, 18173375).
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文献和实验，PD-1/PD-L1这么值钱，大家一定好奇它到底是什么鬼。 PD-1全名是程序性死亡分子1(programmed death-1)是Ishida等[1]通过消减杂交技术于1992年发现的[2]，是CD28家族成员，胞质区含有2个酪氨酸残基，1个位于靠近N端的免疫受体酪氨酸抑制基序(ITIM)中，另一个1个位于靠近C端的免疫受体酪氨酸转化基序(ITSM)中[2]。 PD-L1(CD274)是B7家族成员，是PD-1的配体，属于1型跨膜蛋白，全长290个氨基酸，包含1个IgV样
示意图 (A) 与 6-8 周龄雌性无胸腺裸鼠异种移植肿瘤生长情况 (B)[1]。 表观基因组重编程与转录因子转换是研究揭示的第一个关键机制。H3K27ac 作为活跃增强子/启动子的标志，其 ChIP-seq 分析显示，禁食联合 TMX 治疗导致了肿瘤表观基因组的广泛重编程。具体而言，禁食使 TMX 治疗下的肿瘤丧失了 289个 H3K27ac 富集区域，同时获得了 291 个新的 H3K27ac 位点。深入分析发现，丢失的 H3K27ac 位点显著富集于 AP-1 转录因子家族成员
周期示意图 (A) 与 6-8 周龄雌性无胸腺裸鼠异种移植肿瘤生长情况 (B)[1]。 表观基因组重编程与转录因子转换是研究揭示的第一个关键机制。H3K27ac 作为活跃增强子/启动子的标志，其 ChIP-seq 分析显示，禁食联合 TMX 治疗导致了肿瘤表观基因组的广泛重编程。具体而言，禁食使 TMX 治疗下的肿瘤丧失了 289个 H3K27ac 富集区域，同时获得了 291 个新的 H3K27ac 位点。深入分析发现，丢失的 H3K27ac 位点显著富集于 AP-1 转录因子家族成员 (包括
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