- ￥980 - 6000
- Proteintech
- 17435-1-AP
- 2025年10月13日
- WB, IHC, IF/ICC, IF-P, ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human TF protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, IF-P, ELISA
- 靶点：
Transferrin/TF
- 抗体英文名：
Transferrin/TF Polyclonal antibody
- 抗体名：
Transferrin/TF Polyclonal antibody
- 规格：
100ul/500ul/1ml
| 规格： | 100ul | 产品价格： | ￥980.0 |
|---|---|---|---|
| 规格： | 500ul | 产品价格： | ￥4000.0 |
| 规格： | 1ml | 产品价格： | ￥6000.0 |
经过测试的应用
| Positive WB detected in | human urine sample, mouse kidney tissue, mouse spleen tissue, rat kidney tissue |
| Positive IHC detected in | human hepatocirrhosis tissue, human placenta tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human placenta tissue |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17435-1-AP targets Transferrin/TF in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, ELISA |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, monkey, bovine, duck |
| 免疫原 |
CatNo: Ag11504 Product name: Recombinant human TF protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 347-698 aa of BC059367 Sequence: EGTCQEAPTDECKPVKWCALSHHERLKCDEWSVNSVGKIECVSAETTEDCIAKIMNGEADAMSLDGGFVYIAGKCGLVPVLAENYNKSDNCEDTPEAGYFAVAVVKKSASDLTWDNLKGKKSCHTAVGRTAGWNIPMGLLYNKINHCRFDEFFSEGCAPGSKKDSSLCKLCMGSGLNLCEPNNKEGYYGYTGAFRCLVEKGDVAFVKHQTVPQNTGGKNPDPWAKNLNEKDYELLCLDGTRKPVEEYANCHLARAPNHAVVTRKDKEACVHKILRQQQHLFGSNVTDCSGNFCLFRSETKDLLFRDDTVCLAKLHDRNTYEKYLGEEYVKAVGNLRKCSTSSLLEACTFRRP |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | transferrin |
| 别名 | TF, transferrin, Siderophilin, Serotransferrin, PRO2086 |
| 计算分子量 | 698 aa, 77 kDa |
| 观测分子量 | 77 kDa |
| GenBank蛋白编号 | BC059367 |
| 基因名称 | TF |
| Gene ID (NCBI) | 7018 |
| RRID | AB_2035023 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P02787 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
What is the tissue specificity of TF?
Transferrin is synthesized mainly in the liver.
What is the calculated molecular weight of TF?
77 kDa
How many iron atoms can bind to TF?
Each of the two lobes of TF has one high-affinity Fe(III) binding site. Since binding of iron and bicarbonate
releases free H+ ions, affinity decreases in acidic conditions, in which TF also remains bound to its receptor.
What is the primary role of TF?
TF serves as a serum metal-binding protein, playing a key role in iron metabolism and delivery for erythropoiesis.
What is the role of TF in human diseases?
TF deficiency results in an exhibition of congenital atransferrinemia (hypotransferrinemia) in which there is a
decrease in hepcidin expression, causing increased iron levels.
Is TF essential for iron delivery?
Congenital atransferrinemia results in an iron overload in some tissues, such as the liver, suggesting that while TF is not essential for iron delivery to all organs, it is needed for iron level regulation.
In what circumstances do TF levels decrease?
Inflammation and iron overload both can cause a decline in TF levels.
How does TF affect hormonal balance?
TF positively regulates expression of hepcidin, a peptide hormone synthesized by the liver.
How does TF deficiency cause increased iron levels?
TF regulates hepcidin, which in turn is a negative regulator of intestinal iron absorption, resulting in TF
deficiency causing both anemia, and, paradoxically, increased iron levels in some tissues.
PMIDs: 23046645, 9635730, 22294463, 24589273
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB | Nature 7-Dehydrocholesterol dictates ferroptosis sensitivityAuthors - Yaxu Li |
| human | IF | Nat Neurosci Myelin-axon interface vulnerability in Alzheimer's disease revealed by subcellular proteomics and imaging of human and mouse brainAuthors - Yifei Cai |
| WB | Nat Commun ACSS2 protects against alcohol-induced hepatocyte ferroptosis through regulation of hepcidin expressionAuthors - Mengyao Wang | |
| human | WB | Mol Cell Maintaining Iron Homeostasis Is the Key Role of Lysosomal Acidity for Cell Proliferation.Authors - Ross A Weber |
| human,mouse | WB | Sci Adv The adipocyte-enriched secretory protein tetranectin exacerbates type 2 diabetes by inhibiting insulin secretion from β cellsAuthors - Fen Liu |
| human | WB | Dev Cell Lactylation of LSD1 is an acquired epigenetic vulnerability of BRAFi/MEKi-resistant melanomaAuthors - Aicun Li |
Polyclonal and Monoclonal Antibodies
Maintenance of the cells: Stock solutions: IMDM : Fetal Calf Serum- Transferrin: Iron saturated. 1000X stock = 1 mg/ml HT supps: 50X from Sigma H0137 ( Store at -20 C ). 2-Mercaptoethanol: 1000X stock (5 x 10 -2). (Store
因此，综合来说，EarthOx的GST标签抗体 （货号#E022040）是一款同时具有高特异性、高灵敏度、多应用类型以及性价比的GST标签抗体。同时，我们也提供其他品牌的GST标签抗体，如Proteintech的GST多克隆抗体 ――GST PolyClonal Antibody（货号#10000-0-AP）以及Abcam、Santa等多种GST标签抗体产品。更多GST标签抗体以及其他如His、HA、Myc、GFP、Flag等其他标签抗体，请电话垂询。美国EarthOx品牌是一家提供高性价比免疫学和细胞
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