SNAI1 多克隆抗体 13099-1-AP产品图
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SNAI1 多克隆抗体 13099-1-AP

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  • ¥1350 - 3000
  • Proteintech已认证
  • 13099-1-AP
  • 2025年10月13日
  • WB, IP, IHC, ELISA
  • Rabbit
  • human, mouse, rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      Recombinant human SNAI1 protein

    • 亚型

      IgG

    • 保存条件

      Store at -20°C. Stable for one year after shipment.

    • 克隆性

      Polyclonal

    • 标记物

      Unconjugated

    • 适应物种

      human, mouse, rat

    • 保质期

      详见产品包装

    • 库存

      充足

    • 宿主

      Rabbit

    • 应用范围

      WB, IP, IHC, ELISA

    • 靶点

      SNAI1

    • 抗体英文名

      SNAI1 Polyclonal antibody

    • 抗体名

      SNAI1 Polyclonal antibody

    • 规格

      50ul/100ul/150ul

    规格:50ul产品价格:¥1350.0
    规格:100ul产品价格:¥2200.0
    规格:150ul产品价格:¥3000.0

    经过测试的应用

    Positive WB detected inmouse heart tissue, PC-3 cells, BxPC-3 cells, human heart tissue, COLO 320 cells, MCF-7 cells
    Positive IP detected inMCF-7 cells
    Positive IHC detected inhuman stomach cancer tissue
    Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

    推荐稀释比

    应用推荐稀释比
    Western Blot (WB)WB : 1:500-1:1000
    Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
    Immunohistochemistry (IHC)IHC : 1:50-1:500
    It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
    Sample-dependent, Check data in validation data gallery.

    产品信息

    13099-1-AP targets SNAI1 in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

    经测试应用 WB, IP, IHC, ELISA
    文献引用应用WB, IHC, IF, IP, CoIP, ChIP
    经测试反应性 human, mouse, rat
    文献引用反应性human, mouse, rat, goat
    免疫原

    CatNo: Ag3723

    Product name: Recombinant human SNAI1 protein

    Source: e coli.-derived, PGEX-4T

    Tag: GST

    Domain: 1-264 aa of BC012910

    Sequence: MPRSFLVRKPSDPNRKPNYSELQDSNPEFTFQQPYDQAHLLAAIPPPEILNPTASLPMLIWDSVLAPQAQPIAWASLRLQESPRVAELTSLSDEDSGKGSQPPSPPSPAPSSFSSTSVSSLEAEAYAAFPGLGQVPKQLAQLSEAKDLQARKAFNCKYCNKEYLSLGALKMHIRSHTLPCVCGTCGKAFSRPWLLQGHVRTHTGEKPFSCPHCSRAFADRSNLRAHLQTHSDVKKYQCQACARTFSRMSLLHKHQESGCSGCPR

    宿主/亚型 Rabbit / IgG
    抗体类别 Polyclonal
    产品类型 Antibody
    全称 snail homolog 1 (Drosophila)
    别名 Protein sna, Protein snail homolog 1, SLUGH2, SNA, SNAH, SNAI1, snail homolog 1 (Drosophila), SNAIL1, Zinc finger protein SNAI1
    计算分子量 264 aa, 29 kDa
    观测分子量29-35 kDa
    GenBank蛋白编号BC012910
    基因名称 SNAI1
    Gene ID (NCBI) 6615
    RRIDAB_2191756
    偶联类型 Unconjugated
    形式Liquid
    纯化方式Antigen affinity purification
    UNIPROT IDO95863
    储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
    储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

    背景介绍

    SNAI1, a member of SNAI1 family of protein, participates in the epithelial to mesenchymal transition(EMT) and formation and maintenance of embryonic mesoderm. The snail family share a common structural, that a highly conserved C-terminal region containing a zinc finger transcription factor. SNAI1 interacts with other corepressor, such as Ajuba, PRMT5 and SIN3a or HDAC1 and 2, to repress the target gene. As the phosphorylation modification of SNAI1 protein, the range of molecular weight of SNAI1 is about 25-30 kDa (PMID: 22276203 ). Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs.

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    图标文献和实验
    该产品被引用文献
    [1]Brexpiprazole inhibits EMT and migration of colorectal cancer cells by downregulating the SREBP1/SNAI1 signaling pathway.
    PMID:41626183Front Oncol2026-01-15
    [2]Identification of PLXNC1 as a novel biomarker for consensus molecular subtype 4 in colorectal cancer.
    PMID:42004227Genes Dis2025-12-11
    [3]Placental mesenchymal stem cell-derived interleukin-6 promotes neuroblastoma progression.
    PMID:40948535Am J Cancer Res2025-08
    [4]Thermosensitive hydrogel composite with si-Cx43 nanoparticles and anti-VEGF agent for synergistic treatment of diabetic retinopathy.
    PMID:40528841Mater Today Bio2025-05-29
    [5]TMBIM1 promotes epithelial mesenchymal transition by accelerating autophagic degradation of E-cadherin in glioblastoma.
    PMID:40394049Sci Rep2025-05
    [6]Downregulation of CPT2 promotes proliferation and migration through the TNFα/NF-κB pathway in cholangiocarcinoma.
    PMID:40386589J Gastrointest Oncol2025-04-30
    [7]eNAMPT/Ac-STAT3/DIRAS2 Axis Promotes Development and Cancer Stemness in Triple-Negative Breast Cancer by Enhancing Cytokine Crosstalk Between Tumor-Associated Macrophages and Cancer Cells.
    PMID:40083697Int J Biol Sci2025-02-18
    [8]Sinensetin suppresses breast cancer cell progression via Wnt/β-catenin pathway inhibition.
    PMID:38410229Transl Cancer Res2024-01-31
    [9]Timosaponin AIII promotes non-small-cell lung cancer ferroptosis through targeting and facilitating HSP90 mediated GPX4 ubiquitination and degradation.
    PMID:37056925Int J Biol Sci2023-02-27
    [10]WNT5A promotes the metastasis of esophageal squamous cell carcinoma by activating the HDAC7/SNAIL signaling pathway.
    PMID:35595735Cell Death Dis2022-05
    相关实验
    • 有适合免疫沉淀IP的GST抗体吗?

      因此,综合来说,EarthOx的GST标签抗体 (货号#E022040)是一款同时具有高特异性、高灵敏度、多应用类型以及性价比的GST标签抗体。同时,我们也提供其他品牌的GST标签抗体,如Proteintech的GST多克隆抗体 ――GST PolyClonal Antibody(货号#10000-0-AP)以及Abcam、Santa等多种GST标签抗体产品。更多GST标签抗体以及其他如His、HA、Myc、GFP、Flag等其他标签抗体,请电话垂询。美国EarthOx品牌是一家提供高性价比免疫学和细胞

    • 抗体选择

      实验,请根据说明书列出已验证的种属来选择适合你实验的抗体。   (4)样本蛋白的结构性质。了解样本蛋白的结构性质有助于选择最合适的抗体,待测样本蛋白的结构域和样本在提取和处理过程中是否会变性,蛋白空间构象的改变,会影响抗体的免疫亲和反应。   (5)单多克隆抗体的选择。市面上的抗体还是以多克隆抗体为主,一般单克隆抗体特异性强,但亲和力相对小,检测抗原灵敏度相对就低;而多克隆抗体特异性稍弱,但抗体的亲和力强,灵敏度高,但易出现非特异性染色(可以通过封闭等避免

    • 石蜡切片免疫组织化学染色

      发生,最好在特异性抗体处理切片之前,选择与二抗种属相同的非免疫血清封闭电荷,阻止一抗与之结合,抑制非特异性背景着色。常用方法是用2%~10%羊血清或2%~5%牛血清白蛋白在室温下作用10~30分钟。但应注意此种结合不牢固,所以最好不要冲洗,倾去余液直接加一抗。SABC或SP免疫组织化学试剂盒中常配有非免疫血清。 7.第一抗体孵育 滴加特异性一抗于切片上,4℃孵育24~48小时,或室温孵育过夜,也可在37~C孵育1~2小时。之后,用PBS冲洗3次,每次5分钟。一抗分为多克隆抗体和单克

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    文献支持
    SNAI1 多克隆抗体 13099-1-AP
    ¥1350 - 3000