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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Mouse Transferrin Receptor / TFRC / CD71 Protein (ECD,His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
1.00 mg/50.00 µg/100.00 µg
| 规格: | 1.00 mg | 产品价格: | ¥26570.0 |
|---|---|---|---|
| 规格: | 50.00 µg | 产品价格: | ¥2570.0 |
| 规格: | 100.00 µg | 产品价格: | ¥4520.0 |
蛋白名称:Transferrin Receptor/TFRC蛋白, Transferrin Receptor/TFRC protein
蛋白构建:A DNA sequence encoding the extracellular domain of mouse TFRC (Q62351) (Cys 89-Phe 763) was fused with a polyhistidine tag at the N-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 92 % as determined by SDS-PAGE
蛋白活性:0
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:His
蛋白分子量:The recombinant mouse TFRC comprises 694 amino acids and predicts a molecular mass of 78.3 kDa. The apparent molecular mass of mouse TFRC is approximately 80 kDa in SDS-PAGE under reducing conditions.
蛋白NP号:Q62351
蛋白氨基酸序列:Cys89-Phe763
蛋白标签:N-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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- 作者
- 内容
- 询问日期
文献和实验2, Fan K, et al. Questions about horse spleen ferritin crossing the blood brain barrier via mouse transferrin receptor 1.Protein & cell, PubMed ID: 28993977
3, Wouters Y, et al. Identification and in vivo characterization of a brain-penetrating nanobody.Fluids and barriers of the CNS, PubMed ID: 33054787
4, Joly S, et al. Nogo-A neutralization in the central nervous system with a blood-brain barrier-penetrating antibody.Journal of controlled release : official journal of the Controlled Release Society, PubMed ID: 38154541
ELISA-based assay for scatchard analysis of ligand-receptor interactions
transferrin to its receptors on mouse fibroblasts and yieldedK d values similar to those obtained with other published methods. The assay is sensitive, rapid, and also convenient, because aliquots of semi-intact cells can be stored frozen. The perforated
Polyclonal and Monoclonal Antibodies
Maintenance of the cells: Stock solutions: IMDM : Fetal Calf Serum- Transferrin: Iron saturated. 1000X stock = 1 mg/ml HT supps: 50X from Sigma H0137 ( Store at -20 C ). 2-Mercaptoethanol: 1000X stock (5 x 10 -2). (Store
Purification of Endotoxin Free mAbs
is applicable to most rat and mouse IgG antibodies (not IgM), although individual monoclonal antibodies may require modification of either the pH or ionic strength for optimal purification. Cation exchange using Fast Flow S-Sepharose (Sigma S 1264) at pH
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