重组人 IGFBP6 / IBP-6 蛋白 (His标签)(产品说明)
蛋白名称:Human IGFBP6 / IBP-6 Protein (His Tag)
蛋白构建:A DNA sequence encoding the human IGFBP6 (P24592) (Met 1-Gly 240) was fused with a polyhistidine tag at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 96 % as determined by SDS-PAGE
蛋白活性:1. Measured by its ability to bind human IGF1 (Cat:10598-H24E) in functional ELISA. 2. Measured by its ability to bind human IGF2 (Cat:13032-H24E) in functional ELISA. 3. Measured by its ability to inhibit the biological activity of IGFII on MCF7 human breast adenocarcinoma cells (Karey, K.P. et al. (1988) Cancer Research 48:4083.). The ED50 for this effect is typically 1-5 μg/mL in the presence of 14 ng/mL human IGFII.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Arg 28
蛋白分子量:The recombinant human IGFBP6 consists of 224 amino acids and has a predicted molecular mass of 23.9 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhIGFBP6 is approximately 36 kDa due to glycosylation.
蛋白NP号:P24592
蛋白氨基酸序列:Met1-Gly240
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.