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- Sino Biological
- 北京
- 10326-H08H
- 2025年08月12日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human HMGB1 / HMG1 Protein (His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
1.00 mg/50.00 µg/100.00 µg/500.00 µg
| 规格： | 1.00 mg | 产品价格： | ￥24150.0 |
|---|---|---|---|
| 规格： | 50.00 µg | 产品价格： | ￥2310.0 |
| 规格： | 100.00 µg | 产品价格： | ￥4520.0 |
| 规格： | 500.00 µg | 产品价格： | ￥16740.0 |
蛋白名称：Human HMGB1 / HMG1 Protein (His Tag)
蛋白构建：A DNA sequence encoding the human HMGB1 protein (NP_002119.1) (Met 1-Glu 215) was fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 93 % as determined by SDS-PAGE
蛋白活性：Measured by its binding ability in a functional ELISA. Immobilized human HMGB at 2 μg/ml (100 μl/well) can bind human AGER. The EC50 of human AGER is 0.27 μg/ml.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Met 1
蛋白分子量：The recombinant human HMGB1 consists of 226 amino acids and has a predicted molecular mass of 26.3 kDa. As a result of glycosylation, the apparent molecular mass of rhHMGB1 is approximately 30-34 kDa in SDS-PAGE under reducing conditions.
蛋白NP号：NP_002119.1
蛋白氨基酸序列：Met1-Glu215
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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- 作者
- 内容
- 询问日期
文献和实验2, Xu YF, et al. High-mobility group box 1 expression and lymph node metastasis in intrahepatic cholangiocarcinoma.World journal of gastroenterology, PubMed ID: 25805932
3, Chen Z, et al. Doxorubicin conjugated with nanodiamonds and in free form commit glioblastoma cells to heterodromous fates.Nanomedicine (London, England), PubMed ID: 30676239
4, Yamamoto K, et al. A top-down approach to uncover the hidden ligandome of low-density lipoprotein receptor-related protein 1 in cartilage.Matrix biology : journal of the International Society for Matrix Biology, PubMed ID: 36028175
5, You Q, et al. HMGB1 Release Induced by EV71 Infection Exacerbates Blood-Brain Barrier Disruption via VE-cadherin Phosphorylation.Virus research, PubMed ID: 37832655
6, Shen P, et al. Isolation and microbial transformation of tea sapogenin from seed pomace of Camellia oleifera with anti-inflammatory effects.Chinese journal of natural medicines, PubMed ID: 38553195
Chromatin Interaction Analysis Using Paired‐End Tag Sequencing
Analysis using Paired?End Tag sequencing (ChIA?PET) is a technique developed for large?scale, de novo analysis of higher?order chromatin structures. Cells are treated with formaldehyde to cross?link chromatin interactions, DNA segments bound by protein
.3 ). Concatamers of tags are cloned and sequenced to yield a STAGE library. Tags in the library represent DNA fragments that were occupied by the DNA?binding protein, and mapping these tag sequences to the genome identifies the binding loci of the DNA?binding
Identifying Protein Interactions by Hydroxyl‐Radical Protein Footprinting
Basic Protocol 1: Generation of an Active Recombinant Protein That is End‐Labeled Basic Protocol 2: Hydroxyl Radical Cleavage of Protein in Absence and Presence of Ligand Basic Protocol 3: Analyze Gel
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