DYKDDDDK Tag Antibody (Binds t
o same epitope as Sigma's Anti-FLAG M2 Antibody) (HRP Conjugate)__®- 询价
- Cell Signaling Technology
- 2044
- USA
- 2025年09月10日
- W
- Rabbit
- All
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG M2 Antibody) (HRP Conjugate)__®
- 抗原：
synthetic DYKDDDDK peptide
- 应用范围：
W
- 宿主：
Rabbit
- 适应物种：
All
- 供应商：
CST
- 级别：
详见MSDS文件
- 库存：
大量
- 保质期：
详见说明书
- 标记物：
HRP
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.
| Applications | Reactivity | Sensitivity | Source |
|---|---|---|---|
| W | All | Transfected Only | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | DYKDDDDK Tag Antibody (HRP Conjugate) detects exogenously expressed DYKDDDDK proteins in cells. The antibody recognizes the DYKDDDDK peptide (the same epitope recognized by Sigma's Anti-FLAG® antibodies) fused to either the amino or carboxy terminus of targeted proteins. The binding specificity of this antibody is NOT dependent on the presence of divalent metal cations. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic DYKDDDDK peptide. Antibodies are purified by protein A and peptide affinity chromatography. The antibody was conjugated to HRP under optimal conditions. Western Blotting
Western blot analysis of extracts from COS cells, untransfected (-) or transfected with DYKDDDDK-tagged Fox03 (+), using DYKDDDDK Tag Antibody (HRP Conjugate). |
| Background | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion (1). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
Anti-FLAG® is a registered trademark of Sigma-Aldrich. For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Multiple tandem epitope tagging for enhanced detection of protein expressed in mammalian cells
that can be detected by anti-FLAG monoclonal antibody. In the present study, we have examined the detection sensitivity of a protein fused to three tandem FLAG epitopes by Western blot analysis, immunoprecipitation, and immunohistochemical analysis using anti-FLAG� M2
and then centrifuge at 14,000 RPM for 3 minutes. Save 20µl of the elutes as (Flag-Input). Next, take the remaining ~80µl of elute to clean tubes containing 40µl of EZVIEW™ Red anti-Flag M2 affinity gel beads (Sigma™) which have been pre-treated with a wash in 250µl
Strep/FLAG Tandem Affinity Purification (SF‐TAP) to Study Protein Interactions
dry milk in TBS (see recipe for TBS) containing 0.1% (v/v) Tween 20 Anti‐FLAG M2 antibody (Sigma‐Aldrich
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