VEGF Receptor 2 Antibody

2472:

Western Blotting

VEGF Receptor 2 Antibody detects endogenous levels of VEGF-2 receptors. It does not cross-react with other members of VEGF receptor family .

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy-terminal sequence of human VEGF receptor 2. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from PAE cells overexpressing VEGF receptor 1 and chimeric VEGF receptor 2 respectively, using VEGF Receptor 2 Antibody. CKR/PAE cells overexpress chimeric receptors containing M-CSF receptor extracullular domain and VEGF Receptor 2 transmembrane and cytoplasmic domains (7).

Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, GRB2, PI3 kinase, NCK, and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). Phosphorylation at Tyr1212 provides a docking site for GRB2 binding and phospho-Tyr1175 binds the p85 subunit of PI3 kinase and PLCγ, as well as Shb (1,4,5). Signaling from VEGFR2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo (6-8).

1. Meyer, M. et al. (1999) EMBO J 18, 363-74.
2. Dougher-Vermazen, M. et al. (1994) Biochem Biophys Res Commun 205, 728-38.
3. Kroll, J. and Waltenberger, J. (1997) J Biol Chem 272, 32521-7.
4. Takahashi, T. et al. (2001) EMBO J 20, 2768-78.
5. Holmqvist, K. et al. (2004) J Biol Chem 279, 22267-75.
6. Karkkainen, M.J. and Petrova, T.V. (2000) Oncogene 19, 5598-605.
7. Rahimi, N. et al. (2000) J Biol Chem 275, 16986-92.
8. Claesson-Welsh, L. (2003) Biochem Soc Trans 31, 20-4.

• Diana, D. et al. (2011) J Biol Chem 286, 41680-91. Applications: Western Blotting

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• 2904 VEGF Receptor 2 Control Proteins
• 8065 Human Vascular Endothelial Growth Factor-165 (hVEGF₁₆₅ )
• 3770 Phospho-VEGF Receptor 2 (Tyr1175) (D5B11) Rabbit mAb
• 2478 Phospho-VEGF Receptor 2 (Tyr1175) (19A10) Rabbit mAb
• 2479 VEGF Receptor 2 (55B11) Rabbit mAb
• 3817 Phospho-VEGF Receptor 2 (Tyr1059) (D5A6) Rabbit mAb
• 4991 Phospho-VEGF Receptor 2 (Tyr951) (15D2) Rabbit mAb
• 2477 Phospho-VEGF Receptor 2 (Tyr1212) (11A3) Rabbit mAb
• 2471 Phospho-VEGF Receptor 2 (Tyr951) Antibody
• 2474 Phospho-VEGF Receptor 2 (Tyr996) Antibody
• 2476 Phospho-VEGF Receptor 2 (Tyr951) (7H11) Mouse mAb
• 2893 VEGF Receptor 1 Antibody
• 2638 VEGF Receptor 3 (C28G5) Rabbit mAb
• 2485 VEGF Receptor 3 Antibody
• 2463 VEGF-B Antibody
• 2445 VEGF-C Antibody
• 7071 Phototope^® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

For Research Use Only. Not For Use In Diagnostic Procedures.