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100 µg
蛋白名称:Ephrin B2蛋白, Ephrin B2 protein
蛋白构建:A DNA sequence encoding the human EFNB2 (NP_004084.1) extracellular domain (Met 1-Ala 229) was expressed, fused with a C-terminal polyhistidine tag.
表达宿主:HEK293 Cells
蛋白纯度:> 97 % as determined by SDS-PAGE
蛋白活性:Measured by its binding ability in a functional ELISA . Immobilized human EFNB2 at 2 μg/ml (100 μl/well) can bind human EphB4 with a linear ranger of 1.56-12.5 ng/ml .
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Ile 28
蛋白分子量:The recombinant human EFNB2 consists of 213 amino acids after removal of the signal peptide and predicts a molecular mass of 23.6 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh EFNB2 is approximately 35-40 kDa due to glycosylation.
蛋白NP号:NP_004084.1
蛋白氨基酸序列:Met1-Ala229
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验从肌肉直接抽提的肌动球蛋白标准品。通常是将蟾蜍肌肉在韦伯 -艾得萨( Weber- Edsall)溶液( 0.6MKCl, 0.04M KHCO3 0.01M K2 CO3 )中 24小时后抽提。天然肌动蛋白( natural actomyosin),与纯肌球蛋白和肌动蛋白混合的再生肌动球蛋白( reconstituted actomysin)是有区别的。肌球蛋白 B除肌动蛋白和肌球蛋白之外还包括原肌球蛋白、肌钙蛋白、α -辅肌动蛋白等调节蛋白质,表现有对 Ca2 的敏感
B 细胞抗原受体 B细胞抗原受体(BCR)具有抗原结合特异性,在同一个体内,其多样性高达109 ~1012 ,构成容量巨大的BCR库,赋予个体识别各种抗原、产生特异性抗体的巨大潜能。 在成熟B细胞表面,Iga、Igp总是和BCR共同表达,形成BCR-Igα/Igβ复合体,前者识别抗原,后者转导BCR接受的抗原刺激信号。 (一)BCR分子 BCR主要包括mlgM和mlgD,由两条重链
Isolation of Untouched Human B Cells by Depletion of Non-B Cells
-bound cells with a magnet. Discard the bead-bound cells and use the remaining, untouched human B cells for any application. 实验试剂 Mixer allowing both tilting and rotation. Magnets Isolation Buffer: Ca2 and Mg2 free
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