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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
FLT3 (Y591-V592insVDFREYEYD D835Y), Active
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
10 µg
蛋白名称:FLT3 (Y591-V592insVDFREYEYD D835Y), Active
蛋白构建:Recombinant human FLT3 (Y591-V592insVDFREYEYD D835Y) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. This FLT3-ITD mutant has 9 amino acids “VDFREYEYD” repeated between the sites Y591 and V592 within the juxtamembrane domain.
表达宿主:Baculovirus-Insect Cells
蛋白分子量:~76 kDa
蛋白NP号:NM_004119
蛋白氨基酸序列:571-993 (Y591-V592insVDFREYEYD D835Y)
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文献和实验FLT3 Mutations in Acute Myeloid Leukemia
The prevalence of an internal tandem duplication (ITD) of the juxtamembrane domain-coding sequence and a missense mutation of D835 within the kinase domain of the FLT3 gene is 15–35% and 5–10% of adults with acute myeloid leukemia (AML
。 接下来,研究人员在58个AEL病人中(29个N-AEL,9个AML-MRC,13个MDS-AEL,7个普通AEL)进行了GATA2,CEBPA,NPM1和FLT3-ITD的18个突变的Sanger测序验证。结果显示,有42个病人中有17个携带一个突变,25个携带2个突变,6个携带3个突变,3个携带4个突变。其中,CEBPA突变频率为32.7%,GATA2突变频率为22.4%,NPM1突变频率为15.5%,SETBP1突变频率为12.1%,U2AF1突变频率为12.1%。值得注意的是,在AML发现
Generation of Dendritic Cells from Lymphoid Precursors
. Methods have become available to grow DC in culture, facilitating the study of their biological and developmental properties. One such method is described in this chapter to generate DC in vitro from CD34+ Lineage- (Lin- ) CD10+ bone marrow (BM) progenitor
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