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分别对miRNA与mRNA、miRNA与circRNA进行靶向预测，并根据表达趋势取负相关。根据结果找出具有mRNA-miRNA-circRNA关系的mRNA/circRNA对。计算差异基因和差异circRNA间的共表达关系，挑选出互为正相关的基因与circRNA。根据上述分析结果，选出存在于mRNA-miRNA-circRNA关系中，并互为正相关的mRNA/circNA对。这对mRNA/circRNA即可能互为ceRNA，再通过MREs的数量和结合分值评估ceRNA的竞争能力。结果样式如下：
参考文献： Ling Feng et al. Integrated Analysis of Long Noncoding RNA and mRNA Expression Profile in Advanced Laryngeal Squamous Cell Carcinoma. Plos one. 2016.
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文献和实验miRNA and shRNA Expression Vectors Based on mRNA and miRNA Processing
vectors, designated pSM155, and pSM30 that take into consideration of miRNA processing and RNA splicing by placing the miRNA-based artificial miRNA expression cassettes inside of synthetic introns. These vectors significantly improved the expression
Solution Structure of miRNA:mRNA Complex
The use of contemporary nuclear magnetic resonance (NMR) methods in the studies of model systems between microRNA (miRNA) and messenger RNA (mRNA) is reviewed. We describe our studies on structural features of 33-nt RNA model construct
technology to isolate cells from the outer nuclear, inner nuclear, and ganglion cell layers of the retina for mRNA and microRNA (miRNA) expression and epigenetic (DNA methylation) analysis. Here, we describe the methods we have employed for sample preparation
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