• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Solution Structure of miRNA:mRNA Complex

        互联网

        754
        The use of contemporary nuclear magnetic resonance (NMR) methods in the studies of model systems between microRNA (miRNA) and messenger RNA (mRNA) is reviewed. We describe our studies on structural features of 33-nt RNA model construct between let-7 miRNA and lin-41 mRNA at the second binding site. let-7 miRNA inhibits translation of lin-41 gene through formation of two complexes with the target sequence within 3′ untranslated region of lin-41 mRNA in Caenorhabditis elegans . The base pairing, asymmetric internal loops, and adenine bulge in both the complementary sites are important for regulation of gene expression. NMR study on the uniformly 13 C- and 15 N-labeled RNA construct has shown that RNA molecule folds into a stable structure consisting of two stem regions separated by a well-defined asymmetric internal loop. Solution-state NMR can make important contribution toward deeper understanding of assembly, folding, and structural features of miRNA:mRNA complexes.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序