Sample:
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Lane 1: Cerebrum (Rat) Lysate at 40 ug
_x000D_
Lane 2: Stomach (Rat) Lysate at 40 ug
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Primary: Anti-Wnt3a (bs-1700R) at 1/1000 dilution
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Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
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Predicted band size: 39 kD
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Observed band size: 39 kD
- ￥1180 - 2800
- Bioss
- bs-1700R
- 2026年10月13日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-1700R |
| 英文名称 | Wnt3a Rabbit pAb |
| 中文名称 | 信号通路Wnt3a抗体 |
| 英文别名 | MGC119418; MGC119419; MGC119420; Protein Wnt 3a Precursor; Protein Wnt-3a; Wingless type MMTV integration site family member 3A; Wnt 3a; WNT3A protein. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Rat (Human, Mouse, Dog, Pig, Cow, Rabbit, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Wnt3a |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 37 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Developmental Biology > Embryogenesis > Morphogens Neuroscience > Neurology process > Neurogenesis Stem Cells > Signaling Pathways > Wnt > Secreted |
| 亚基 | Interacts with PORCN. Interacts with APCDD1 and WLS. Component of the Wnt-Fzd-LRP5-LRP6 signaling complex that contains a WNT protein, a FZD protein and LRP5 or LRP6. Interacts directly in the complex with LRP6. |
| 亚细胞定位 | Secreted, extracellular space, extracellular matr |
| 组织特异性 | Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate. |
| 翻译后修饰 | Palmitoylation at Ser-209 by PORCN is required for proper trafficking to cell surface, vacuolar acidification is critical to release palmitoylated WNT3A from WLS in secretory vesicles. |
| 相似性 | Belongs to the Wnt family. |
| 功能 | Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This gene is clustered with WNT14 gene, another family member, in chromosome 1q42 region. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
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= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
are needed to reveal the important role of the members of the Wnt signaling pathway in the RA pathogenesis and treatment. Rspo 1 promotes osteoblast differentiation via Wnt signaling pathway. 作者：Sharma AR, Choi BS, Park JM, Lee DH, Lee JE, Kim HS
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
cellular GSK3 and assay asabove, although this may be a very transient effect as we(Fig. 4.3 ) and others (10 , 12) do not see any change in GSK3specific activity beyond 30 min exposure of cells to Wnt. Fig. 4.1. Wnt3a regulates GSK3-mediated
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WNT3A Rabbit pAb(bs-1700R)-50ul/100ul/200ul
￥1180 - 2800
