Tissue/cell: human placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ADFP Polyclonal Antibody, Unconjugated(bs-1164R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
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- bs-1164R
- 2025年10月24日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-1164R |
| 英文名称 | Perilipin-2 Rabbit pAb |
| 中文名称 | 脂肪分化相关蛋白抗体 |
| 英文别名 | ADFP; Adipophilin; Adipose differentiation related protein; Adipose differentiation-related protein; PLIN2; PLIN2_HUMAN; adipophilin; Adipose differentiation related protein; ADRP; MGC10598 |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human ADFP |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 48 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚细胞定位 | Membrane; Peripheral membrane protein. |
| 组织特异性 | Milk lipid globules. |
| 翻译后修饰 | Acylated; primarily with C14, C16 and C18 fatty acids. |
| 相似性 | Belongs to the perilipin family. |
| 功能 | May be involved in development and maintenance of adipose tissue. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2011] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Sample: Liver (Mouse) Lysate at 40 ug
Primary: Anti-ADFP (bs-1164R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD
Primary: Anti-ADFP (bs-1164R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD
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文献和实验该产品被引用文献
[IF={{ 8.47 }}] {Amrutkar, Manoj, et al. "Genetic Disruption of Protein Kinase STK25 Ameliorates Metabolic Defects in a Diet-Induced Type 2 Diabetes Model."Diabetes (2015).} {Other} {="Mouse"}
[IF={{ 7.097 }}] {Yilan Shen et al. VEGF-B antibody and interleukin-22 fusion protein ameliorates diabetic nephropathy through inhibiting lipid accumulation and inflammatory responses.} {IF} {mouse}
[IF={{ 3.7 }}] {Lei Zhang. et al. Effect of Rab18 on liver injury and lipid accumulation by regulating perilipin 2 and peroxisome proliferator-activated receptor gamma in non-alcoholic fatty liver disease. J GASTROEN HEPATOL. 2024 Jul;:} {ICC} {Mouse}
[IF={{ 2.2 }}] {Liu et al. Oxidized low-density lipoprotein activates adipophilin through ERK1/2 signal pathway in RAW264.7 cells. (2010) Acta.Biochim.Biophys.Sin.(Shanghai. 42:635-45} {WB} {Mouse}
[IF={{ 1.851 }}] {Liu Q et al. Inhibitory effect of 17β‑estradiol on triglyceride synthesis in skeletal muscle cells is dependent on ESR1 and not ESR2. Molecular Medicine Reports.2019,19: 5087-5096 .} {Other} {Rat&Mouse}
相关实验
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
/3000 进口分装 F1162 小鼠基质金属蛋白酶抑制剂-2(mouse TIMP-2) ELISA 48T/96T 2000/3000 进口分装 F1163 ★ 小鼠肿瘤坏死因子- a (mouse TNF- a ) ELISA 48T/96T 1300/2300 进口分装 F1164 小鼠可溶性肿瘤坏死因子- 受体 (mouse sTNF-R) ELISA 48T/96T 1300/2300 进口分装 F1165 小鼠
/3000 进口分装 F1162 小鼠基质金属蛋白酶抑制剂-2(mouse TIMP-2) ELISA 48T/96T 2000/3000 进口分装 F1163 ★ 小鼠肿瘤坏死因子- a (mouse TNF- a ) ELISA 48T/96T 1300/2300 进口分装 F1164 小鼠可溶性肿瘤坏死因子- 受体 (mouse sTNF-R) ELISA 48T/96T 1300/2300 进口分装 F1165 小鼠
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Perilipin-2 Rabbit pAb(bs-1164R)-50ul/100ul/200ul
￥1180 - 2800
