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- Bioss
- bs-13108R
- 2026年10月13日
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- 技术资料
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-13108R |
| 英文名称 | ESRP2 Rabbit pAb |
| 中文名称 | 上皮粘连调控蛋白ESRP2抗体 |
| 英文别名 | Epithelial splicing regulatory protein 2; ESRP 2; ESRP2; ESRP2_HUMAN; FLJ21918; FLJ22248; RBM35B; RNA binding motif protein 35A; RNA binding motif protein 35B; RNA binding protein 35B; RNA-binding motif protein 35B; RNA-binding protein 35B. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat, Dog, Cow) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human ESRP2 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 78 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > Chromatin Binding Proteins > DNA / RNA binding Epigenetics and Nuclear Signaling > DNA / RNA > RNA Processing > Splicing Tags & Cell Markers > Cell Type Markers > Epi / Endo-thelial |
| 亚细胞定位 | Nucleus. |
| 组织特异性 | Epithelial cell-specific. |
| 相似性 | Belongs to the ESRP family. _x000D_ Contains 3 RRM (RNA recognition motif) domains. |
| 功能 | mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. Specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2. Also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Acts by directly binding specific sequences in mRNAs. Binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | ESPR2 is an epithelial cell-type-specific splicing regulator (Warzecha et al., 2009 [PubMed 19285943]).[supplied by OMIM, Aug 2009] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Paraformaldehyde-fixed, paraffin embedded (Mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ESRP2) Polyclonal Antibody, Unconjugated (bs-13108R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Sample:
_x000D_ A431(Human) Cell Lysate at 30 ug
_x000D_ Hela(Human) Cell Lysate at 30 ug
_x000D_ HepG2(Human) Cell Lysate at 30 ug
_x000D_ Primary: Anti-ESRP2 (bs-13108R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 78 kD
_x000D_ Observed band size: 78 kD
_x000D_ A431(Human) Cell Lysate at 30 ug
_x000D_ Hela(Human) Cell Lysate at 30 ug
_x000D_ HepG2(Human) Cell Lysate at 30 ug
_x000D_ Primary: Anti-ESRP2 (bs-13108R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 78 kD
_x000D_ Observed band size: 78 kD
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ESRP2 | RBM35B Rabbit pAb(bs-13108R)-50ul/100ul/200ul
￥1180 - 2800
