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HBV | HBsAg Goat pAb(bs-1557G)

-50ul/100ul/200ul

价格￥1180 - 2800

品牌Bioss
货号bs-1557G
更新日期2026年10月13日

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详细信息
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技术资料

规格：
50ul/100ul/200ul

规格：	50ul	产品价格：	￥1180.0
规格：	100ul	产品价格：	￥1980.0
规格：	200ul	产品价格：	￥2800.0

产品编号	bs-1557G
英文名称	HBsAg Goat pAb
中文名称	山羊抗人乙肝表面抗原抗体（包被）
英文别名	L glycoprotein; L-HBsAg; LHB; Large S protein; Large surface protein; Major surface antigen; S; HBSAG_HBVC5.
产品应用	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000 Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
交叉反应	HBV
抗体来源	Goat
免疫原	Recombinant HBsAg protein
亚型	IgG
性状	Liquid
纯化方法	affinity purified by Protein A
克隆类型	Polyclonal
理论分子量	44 kDa
浓度	1mg/ml
储存液	0.01M PBS (pH7.4) with 0.02% Proclin300.
研究领域	Microbiology > Organism > Virus > DNA Virus > double stranded DNA Virus > Hepatitis B
亚基	Li-HBsAg interacts with capsid protein and with HDV Large delta antigen. Isoform M associates with host chaperone CANX through its pre-S2 N glycan. This association may be essential for M proper secretion.
亚细胞定位	Virion membrane.
翻译后修饰	Isoform M is N-terminaly acetylated at a ratio of 90%, and N-glycosylated at the pre-S2 region._x000D_ Myristoylated.
相似性	Belongs to the orthohepadnavirus major surface antigen family.
功能	The large envelope protein exists in two topological conformations, one which is termed 'external' or Le-HBsAg and the other 'internal' or Li-HBsAg. In its external conformation the protein attaches the virus to cell receptors and thereby initiating infection. This interaction determines the species specificity and liver tropism. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis. The large envelope protein also assumes fusion between virion membrane and endosomal membrane (Probable). In its internal conformation the protein plays a role in virion morphogenesis and mediates the contact with the nucleocapsid like a matrix protein._x000D_ The middle envelope protein plays an important role in the budding of the virion. It is involved in the induction of budding in a nucleocapsid independent way. In this process the majority of envelope proteins bud to form subviral lipoprotein particles of 22 nm of diameter that do not contain a nucleocapsid.
保存条件	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
背景资料

应用	推荐稀释比例
{WB}	{1:500-2000}
{IHC-P}	{1:100-500}
{IHC-F}	{1:100-500}
{IF}	{1:100-500}
{ELISA}	{1:5000-10000}

Measured by its binding ability in a indirect ELISA. Immobilized recombinant HBsAg protein (Cat. bs-41420P) at 2 μg/mL (100 μL/well) can bind Goat Anti-Human HBsAg Antibody, the EC50 is 29.14 ng/mL._x000D_

Sample:
_x000D_ Lane 1: Recombinant HBsAg protein(bs-41420P)
_x000D_ Primary: Anti-HBsAg (bs-1557G) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Donkey anti-Goat IgG at 1/20000 dilution
_x000D_ Predicted band size: 44 kDa
_x000D_ Observed band size: 20 kDa

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