Sample:
Kidney (Mouse) Lysate at 40 ug
Primary: Anti-TMEM49(bs-6740R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kD
Observed band size: 46 kD
- ￥1180 - 2800
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- bs-6740R
- 2025年10月24日
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| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-6740R |
| 英文名称 | TMEM49 Rabbit pAb |
| 中文名称 | 跨膜蛋白49抗体 |
| 英文别名 | Vacuole membrane protein 1; DKFZP566I133; TDC1; TMEM49; Transmembrane protein 49; vmp1; VMP1_HUMAN. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse (Human, Rat, Pig, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human TMEM49 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 46 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cell Death > Autophagy > Autophagosome Cancer > Tumor biomarkers Cardiovascular > Heart > Autophagy > Autophagosome Metabolism > Pathways and Processes > Metabolism processes > Autophagy and mitophagy > Autophagosome |
| 亚基 | Belongs to the VMP1 family. |
| 亚细胞定位 | Endoplasmic reticulum-Golgi intermediate compartment membrane. Cell membrane. Vacuole membrane. Endoplasmic reticulum. |
| 相似性 | Belongs to the VMP1 family. |
| 功能 | Stress-induced protein that, when overexpressed, promotes formation of intracellular vacuoles followed by cell death. May be involved in the cytoplasmic vacuolization of acinar cells during the early stage of acute pancreatitis. Plays a role in the initial stages of the autophagic process through its interaction with BECN1 (By similarity). Involved in cell-cell adhesion. Plays an essential role in formation of cell junctions.Sequence similarities: Belongs to the VMP1 family. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Vacuole membrane protein 1 (VMP1)/TMEM49 is a transmembrane protein localized to intracellular vacuoles and was discovered as a protein that promotes vacuole formation in acinar cells associated with acute pancreatitis (1). Over-expression of VMP1 promotes vacuole formation and subsequent cell death (1). Subsequent studies have shown that VMP1 expression is induced by starvation and the mTOR inhibitor, rapamycin, and can trigger autophagy (2). VMP1 is targeted, along with LC3, to autophagosome membranes (2). Knockdown of VMP1 can inhibit autophagosome formation (2). VMP1 interacts with Beclin-1, a key autophagy protein that activates the Class III PI3 kinase Vps34, which is regulated by a large network of associated proteins (3). VMP1 functions in the degradation and clearance of zymogen-containing vacuoles during experimental pancreatitis (4). During this process, VMP1 interacts with the ubiquitin protease USP9X, suggesting a possible functional link between the molecular machinery of autophagy and the ubiquitin pathway. Orthologues of VMP1 have been reported in C. elegans (known as EPG-3), Drosophila (known as TANGO-5), and Dictyostelium, and have been shown to play a role in membrane trafficking, orgenelle organization, and autophagy (5-7). |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Tissue/cell: mouse pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TMEM49 Polyclonal Antibody, Unconjugated(bs-6740R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TMEM49 Polyclonal Antibody, Unconjugated(bs-6740R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Sample:
Lane 1: Mouse Stomach tissue lysates
Lane 2: Mouse Cerebrum tissue lysates
Primary: Anti-TMEM49 (bs-6740R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kDa
Observed band size: 48 kDa
Lane 1: Mouse Stomach tissue lysates
Lane 2: Mouse Cerebrum tissue lysates
Primary: Anti-TMEM49 (bs-6740R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kDa
Observed band size: 48 kDa
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文献和实验该产品被引用文献
[IF={{ 8.2 }}] {Jia-Gen Cui. et al. Calcium homeostasis imbalance mediates DEHP induced mitochondrial damage in cerebellum and the antagonistic effect of lycopene. SCI TOTAL ENVIRON. 2024 Dec;954:176351} {WB} {Mouse}
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TMEM49 Rabbit pAb(bs-6740R)-50ul/100ul/200ul
￥1180 - 2800
