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OCLN | Occludin Rabbit pAb(bs-

10011R)-50ul/100ul/200ul

价格￥1180 - 2800

品牌Bioss
货号bs-10011R
更新日期2026年10月13日

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规格：
50ul/100ul/200ul

规格：	50ul	产品价格：	￥1180.0
规格：	100ul	产品价格：	￥1980.0
规格：	200ul	产品价格：	￥2800.0

产品编号	bs-10011R
英文名称	Occludin Rabbit pAb
中文名称	紧密连接蛋白抗体
英文别名	Occludin, Occludin-1; Occludin1; Occludin 1; FLJ08163; BLCPMG; FLJ18079; FLJ77961; FLJ94056; MGC34277; Occludin; OCLN; OCLN_HUMAN; Tight junction protein occludin.
产品应用	WB=1:500-2000, Flow-Cyt=2µg/Test Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
交叉反应	Human, Mouse (Rat, Dog, Pig, Cow, Horse, GuineaPig)
抗体来源	Rabbit
免疫原	KLH conjugated synthetic peptide derived from human Occludin
亚型	IgG
性状	Liquid
纯化方法	affinity purified by Protein A
克隆类型	Polyclonal
理论分子量	59 kDa
浓度	1mg/ml
储存液	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究领域	Signal Transduction > Cytoskeleton / ECM > Cell Adhesion > Tight Junctions
亚基	Interacts with TJP1/ZO1 and with VAPA.
亚细胞定位	Membrane; Multi-pass membrane protein. Cell junction, tight junction.
组织特异性	Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis.
翻译后修饰	Phosphorylated upon DNA damage, probably by ATM or ATR. Dephosphorylated by PTPRJ. May be phosphorylated by PKC during translocation to cell-cell contacts.
相似性	Belongs to the ELL/occludin family._x000D_ Contains 1 MARVEL domain.
功能	May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier.
保存条件	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
背景资料	This gene encodes an integral membrane protein which is located at tight junctions. This protein may be involved in the formation and maintenance of the tight junction. The possibility of several alternatively spliced products has been suggested but the full nature of these products has not been described. [provided by RefSeq].

应用	推荐稀释比例
{WB}	{1:500-2000}
{Flow-Cyt}	{2µg/Test}

Blank control: Mouse kidney.
_x000D_ Primary Antibody (green line): Rabbit Anti-Occludin antibody (bs-10011R)
_x000D_ Dilution: 3μg /10^6 cells;
_x000D_ Isotype Control Antibody (orange line): Rabbit IgG .
_x000D_ Secondary Antibody : Goat anti-rabbit IgG-PE
_x000D_ Dilution: 1μg /test.
_x000D_ Protocol
_x000D_ The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed._x000D_

Sample:
_x000D_ Lane 1: A549 (Human) Cell Lysate at 30 ug
_x000D_ Lane 2: HepG2 (Human) Cell Lysate at 30 ug
_x000D_ Lane 3: Hela (Human) Cell Lysate at 30 ug
_x000D_ Primary: Anti-Occludin (bs-10011R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 65/55/38/28 kD
_x000D_ Observed band size: 62/52/40/25 kD

Blank control: MCF7.
_x000D_ Primary Antibody (green line): Rabbit Anti-Occludin antibody (bs-10011R)
_x000D_ Dilution: 1μg /10^6 cells;
_x000D_ Isotype Control Antibody (orange line): Rabbit IgG .
_x000D_ Secondary Antibody : Goat anti-rabbit IgG-FITC
_x000D_ Dilution: 1μg /test.
_x000D_ Protocol
_x000D_ The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed._x000D_

Blank control(blue): 293T(fixed with 2% P‌‌araformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice).
Primary Antibody: Rabbit Anti-Occludin/FITC Conjugated antibody (bs-10011R /FITC), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.
_x000D_

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