Blank control: Mouse kidney.
Primary Antibody (green line): Rabbit Anti-Occludin antibody (bs-10011R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
- ￥1180 - 2800
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- bs-10011R
- 2025年10月24日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-10011R |
| 英文名称 | Occludin Rabbit pAb |
| 中文名称 | 紧密连接蛋白抗体 |
| 英文别名 | Occludin, Occludin-1; Occludin1; Occludin 1; FLJ08163; BLCPMG; FLJ18079; FLJ77961; FLJ94056; MGC34277; Occludin; OCLN; OCLN_HUMAN; Tight junction protein occludin. |
| 产品应用 | WB=1:500-2000, Flow-Cyt=2µg/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat, Dog, Pig, Cow, Horse, GuineaPig) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Occludin |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 59 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Signal Transduction > Cytoskeleton / ECM > Cell Adhesion > Tight Junctions |
| 亚基 | Interacts with TJP1/ZO1 and with VAPA. |
| 亚细胞定位 | Membrane; Multi-pass membrane protein. Cell junction, tight junction. |
| 组织特异性 | Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis. |
| 翻译后修饰 | Phosphorylated upon DNA damage, probably by ATM or ATR. Dephosphorylated by PTPRJ. May be phosphorylated by PKC during translocation to cell-cell contacts. |
| 相似性 | Belongs to the ELL/occludin family.
Contains 1 MARVEL domain. |
| 功能 | May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes an integral membrane protein which is located at tight junctions. This protein may be involved in the formation and maintenance of the tight junction. The possibility of several alternatively spliced products has been suggested but the full nature of these products has not been described. [provided by RefSeq]. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {Flow-Cyt} | {2µg/Test} |
Sample:
Lane 1: A549 (Human) Cell Lysate at 30 ug
Lane 2: HepG2 (Human) Cell Lysate at 30 ug
Lane 3: Hela (Human) Cell Lysate at 30 ug
Primary: Anti-Occludin (bs-10011R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65/55/38/28 kD
Observed band size: 62/52/40/25 kD
Lane 1: A549 (Human) Cell Lysate at 30 ug
Lane 2: HepG2 (Human) Cell Lysate at 30 ug
Lane 3: Hela (Human) Cell Lysate at 30 ug
Primary: Anti-Occludin (bs-10011R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65/55/38/28 kD
Observed band size: 62/52/40/25 kD
Blank control: MCF7.
Primary Antibody (green line): Rabbit Anti-Occludin antibody (bs-10011R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Primary Antibody (green line): Rabbit Anti-Occludin antibody (bs-10011R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control(blue): 293T(fixed with 2% Paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice).
Primary Antibody: Rabbit Anti-Occludin/FITC Conjugated antibody (bs-10011R /FITC), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.
Primary Antibody: Rabbit Anti-Occludin/FITC Conjugated antibody (bs-10011R /FITC), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.
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文献和实验该产品被引用文献
[IF={{ 9.8 }}] {Yanghuan Yu. et al. MiRNA-seq and mRNA-seq revealed the mechanism of fluoride-induced cauda epididymal injury. SCI TOTAL ENVIRON. 2024 Jun;930:172895} {WB} {Mouse}
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[IF={{ 8.6 }}] {Zhiguo Li. et al. Cross-disease characterization of fibroblast heterogeneities and their pathogenic roles in skin inflammation. CLIN IMMUNOL. 2023 Aug;:109742} {IF} {Human}
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Occludin Rabbit pAb(bs-10011R)-50ul/100ul/200ul
￥1180 - 2800
