Tissue/cell: rat brain tissue(left panel was injury,Right panel was normal); 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-8-OHdG Polyclonal Antibody, Unconjugated(bs-1278R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
- Bioss
- bs-1278R
- 2025年10月24日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-1278R |
| 英文名称 | 8-OHdG (DNA/RNA Damage) Rabbit pAb |
| 中文名称 | 8-羟基脱氧鸟苷抗体 |
| 英文别名 | 8-hydroxy-2'-deoxyguanosine; 2'-Deoxy-8-oxoguanosine; 7, 8-Dihydro-8-oxo-2'-deoxyguanosine; 8-Oxo-7, 8-dihydro-2'-deoxyguanosine; 8-Oxo-7, 8-dihydrodeoxyguanosine; 2'-Deoxy-8-oxo-D-guanosine; 8-Oxo-7, 8-dihydro-2μ-deoxyguanosine, 8-Oxo-dG; 2'-Deoxy-8-hydroxyguanosine; |
| 中文别名 | 8-OH-脱氧鸟嘌呤; 8-羟基-2'-脱氧鸟嘌呤; 2'-脱氧-7,8-二氢-8-氧代鸟苷; 8-羟基-2-脱氧鸟苷; 8-羟基脱氧鸟苷; 2'-脱氧-8-氧代鸟嘌呤; 8-氧-2'-脱氧鸟苷; 核酸氧化8-羟基脱氧鸟苷; 8-羟基-2′-脱氧鸟苷; |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Species independent |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated 8-OHdG |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 0.283 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cell Biology > Other Antibodies > Oxidative Stress Epigenetics and Nuclear Signaling > DNA / RNA > DNA / Nucleotides Metabolism > Pathways and Processes > Redox metabolism > Oxidative stress |
| 功能 | Oxoguanine 8 (8-Oxoguanine) is a mutagenic oxidative damage product of guanine. Guanine is the main target for reactive oxygen species in DNA, with 8-oxoguanine being the most frequent base lesion. Thus formation of 8-oxoguanine is an important biomarker of oxidative damage to DNA. It is primarily repaired by the DNA glycosylase OGG1. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | 8-Hydroxydeoxyguanosine (8OHdG) is a modified base that occurs in DNA due to attack by hydroxyl radicals that are formed as byproducts and intermediates of aerobic metabolism and during oxidative stress. There is increasing evidence to support the involvement of free radical reactions in the damage of biomolecules that eventually lead to several diseases in humans, such as atherosclerosis, cerebral and heart ischemia-reperfusion injury, cancer, rheumatoid arthritis, inflammation, diabetes, aging, and neurodegenerative conditions, such as Alzheimer's disease. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
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文献和实验该产品被引用文献
[IF={{ 9.986 }}] {Shotaro Hayashi. et al. Novel ovarian endometriosis model causes infertility via iron-mediated oxidative stress in mice. Redox Biol. 2020 Oct;37:101726} {IHC} {Mouse}
[IF={{ 9.986 }}] {Wei Ou. et al. Hypoxic acclimation improves cardiac redox homeostasis and protects heart against ischemia-reperfusion injury through upregulation of O-GlcNAcylation. Redox Biol. 2021 Jul;43:101994} {IHC} {Rat}
[IF={{ 9.3 }}] {Xu Lu-Lu. et al. Bruton’s tyrosine kinase inhibition ameliorated neuroinflammation during chronic white matter ischemia. J NEUROINFLAMM. 2024 Dec;21(1):1-15} {IF} {Mouse}
[IF={{ 9.261 }}] {Eroglu, Binnur. et al. Dusp26 phosphatase regulates mitochondrial respiration and oxidative stress and protects neuronal cell death. Cell Mol Life Sci. 2022 Apr;79(4):1-28} {IHC} {Mouse}
[IF={{ 9.2 }}] {Jiang Xue. et al. Dioscin Alleviates Periodontitis by Inhibiting NLRP3 Inflammasome Activation via Regulation of K+ Homeostasis and Mitochondrial Function. INT J BIOL SCI. 2024 Jan;20(4):1375-1388} {IF,IHC} {Mouse}
相关实验
Nucleic Acid Programmable Protein Arrays: Versatile Tools for Array‐Based Functional Protein Studies
Ballard, J.L., Peeva, V.K., deSilva, C.J., Lynch, J.L., and Swanson, N.R., 2007. Comparison of Alexa Fluor and CyDye for practical DNA microarray use. Mol. Biotechnol. 36:175‐183.
Generation of Spatio‐Temporally Controlled Targeted Somatic Mutations in the Mouse
. Berton, T.R., Matsumoto, T., Page, A., Conti, C.J., Deng, C.X., Jorcano, J.L., and Johnson, D.G. 2003. Tumor formation in mice with conditional inactivation of Brca1 in epithelial tissues. Oncogene 22:5415
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8-OHdG (DNA/RNA Damage) Rabbit pAb(bs-1278R)-50ul/100ul/200ul
￥1180 - 2800
