PPM1E Rabbit pAb(bs-21063R)-50ul/100ul/200ul

PPM1E Rabbit pAb(bs-21063R)-50

ul/100ul/200ul
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  • ￥1180 - 2800
  • Bioss已认证
  • bs-21063R
  • 2025年10月16日
  • 产品信息以Bioss网站为准
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    • 规格：

      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-21063R
    英文名称PPM1E Rabbit pAb
    中文名称核钙调蛋白依赖性蛋白激酶磷酸酶抗体
    英文别名Ca(2+)/calmodulin-dependent protein kinase phosphatase N; CaMKN; CaMKP-N; CaMKP-nucleus; KIAA1072; Nuclear calmodulin dependent protein kinase phosphatase; Partner of PIX 1; Partner of PIX-alpha; Partner of PIXA; POPX1; PP2CH; Ppm1e; PPM1E_HUMAN; Protein phosphatase 1E; Protein phosphatase Mg2+/Mn2+dependent 1E.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Mouse (Human, Rat, Chicken, Pig, Cow, Horse, Zebrafish, Sheep)
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human PPM1E
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量85 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Cell Biology > Cell Cycle > Kinases/Phosphatases > Phosphatases

    Signal Transduction > Cytoskeleton / ECM > Cytoskeleton > Microfilaments > Actin etc > Actin

    Signal Transduction > Protein Phosphorylation > Ser / Thr Phosphatases

    亚细胞定位Nucleus. Cytoplasm. A truncated form, major form, with the C-terminal part missing, is mostly found in the cytoplasm and a little in the nucleus. The full length, minor form, is found in the nucleus.
    相似性Belongs to the PP2C family.
    Contains 1 PP2C-like domain.
    功能Protein phosphatase that inactivates multifunctional CaM kinases such as CAMK4 and CAMK2 (By similarity). Dephosphorylates and inactivates PAK. May play a role in the inhibition of actin fiber stress breakdown and in morphological changes driven by TNK2/CDC42.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料This gene encodes a member of the PPM family of serine/threonine-protein phosphatases. The encoded protein is localized to the nucleus and dephosphorylates and inactivates multiple substrates including serine/threonine-protein kinase PAK 1, 5'-AMP-activated protein kinase (AMPK) and the multifunctional calcium/calmodulin-dependent protein kinases. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012]

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}

     

    PPM1E Rabbit pAb(bs-21063R)-50
    Sample:
    Olfactory buib(Mouse) Lysate at 40 ug
    Hippocampus (Mouse) Lysate at 40 ug
    Primary: Anti- PPM1E (bs-21063R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 85 kD
    Observed band size: 85 kD
    PPM1E Rabbit pAb(bs-21063R)-50
    Sample:
    Cerebrum (Mouse) Lysate at 40 ug
    Primary: Anti-PPM1E (bs-21063R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 85 kD
    Observed band size: 85 kD
    PPM1E Rabbit pAb(bs-21063R)-50
    Sample:
    Hippocampus (Mouse) Lysate at 40 ug
    Primary: Anti-PPM1E (bs-21063R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 85 kD
    Observed band size: 85 kD
    PPM1E Rabbit pAb(bs-21063R)-50
    P‌‌araformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PPM1E) Polyclonal Antibody, Unconjugated (bs-21063R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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