FoxP3 Rabbit pAb(bs-0269R)-50ul/100ul/200ul产品图
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FoxP3 Rabbit pAb(bs-0269R)-50u

l/100ul/200ul
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  • ¥1180 - 2800
  • Bioss已认证
  • bs-0269R
  • 2025年10月24日
  • 产品信息以Bioss网站为准
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      50ul/100ul/200ul

    规格:50ul产品价格:¥1180.0
    规格:100ul产品价格:¥1980.0
    规格:200ul产品价格:¥2800.0
    产品编号bs-0269R
    英文名称FoxP3 Rabbit pAb
    中文名称叉头蛋白P3抗体
    英文别名Forkhead box protein P3; forkhead box P3; Scurfin; Forkhead box protein P3, C-terminally processed; Forkhead box protein P3 41 kDa form; JM2; AIID; IPEX; PIDX; XPID; DIETER; FOXP3_HUMAN;
    产品应用WB=1:500-2000, IHC-P=1:200-1000, IHC-F=1:200-1000, IF=1:200-1000

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Mouse, Rat
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human FoxP3
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量47 kDa
    实际分子量50-55kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Cell Biology > Cell Cycle > Cell Division > Chromatid Cohesion

    Epigenetics and Nuclear Signaling > Transcription > Domain Families > Forkhead Box > FOXP

    Immunology > Adaptive Immunity > Regulatory T Cells

    亚基Interacts with IKZF3.
    亚细胞定位Nucleus (Potential).
    翻译后修饰Acetylation on lysine residues stabilizes FOXP3 and promotes differentiation of T-cells into induced regulatory T-cells (iTregs) associated with suppressive functions. Deacetylated by SIRT1.
    相似性Contains 1 C2H2-type zinc finger.
    Contains 1 fork-head DNA-binding domain.
    功能Probable transcription factor. Plays a critical role in the control of immune response.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008].

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:200-1000}
    {IHC-F}{1:200-1000}
    {IF}{1:200-1000}

     

    产品细节图片1
    P‌‌araformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FoxP3 Polyclonal Antibody, Unconjugated (bs-0269R) at 1:1000 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    产品细节图片2
    P‌‌araformaldehyde-fixed, paraffin embedded Mouse Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FoxP3 Polyclonal Antibody, Unconjugated (bs-0269R) at 1:1000 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    产品细节图片3
    P‌‌araformaldehyde-fixed, paraffin embedded Rat Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FoxP3 Polyclonal Antibody, Unconjugated (bs-0269R) at 1:1000 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    产品细节图片4
    25 ug total protein per lane of various lysates (see on figure) probed with FoxP3 polyclonal antibody, unconjugated (bs-0269R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

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    图标文献和实验
    相关实验
    • Immunochemical Detection of a Fluorophore Derived from the Lipid Peroxidation Product 4-Hydroxy-2-Nonenal and Lysine

      = NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific

    • 手把手课程之 IHC 关键操作步骤

      ,EDTA 缓冲液适用于多数磷酸化酪氨酸特异性抗体,而柠檬酸盐缓冲液适用于其他多数抗体。每天都需制备新鲜的 1X 溶液。抗体:hospho-HER3/ErbB3 (Tyr1289) (D1B5) Rabbit mAb #2842样本:蜡包埋人类肺部肿瘤抗原修复缓冲液:柠檬酸盐缓冲液(左)或者 EDTA 缓冲液(右)煮沸装置载玻片在所推荐的缓冲液中加热煮沸一段时间后,才可引起抗原修复。该步骤通常在微波炉或高压锅中进行。虽然,一些研究人员也使用水浴锅,但是,因为该步骤中所选择的装置对染色存在积极或消极的影响

    • 【热门靶点解析】TGF-β信号通路+重要作用+药物研发!(建议收藏)

      -β1在免疫调控中具有双重作用:在IL-2存在下,高浓度TGF-β1促进CD4+T细胞分化为Foxp3+ iTreg细胞,维持免疫耐受;低浓度时则与IL-6或IL-21协同,通过STAT3信号诱导致病性Th17分化。其浓度依赖性效应还体现于抑制Th17特征或促进IL-23R表达。肿瘤微环境中,TGF-β1可诱导Treg分化,抑制CD8+ T细胞功能,助力肿瘤免疫逃逸。 2.TGF-β1刺激成纤维细胞增殖 TGF-β1可促进成纤维细胞增殖、分泌胶原蛋白等细胞外基质,并抑制其降解,参与组织修复与纤维化。研究

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    FoxP3 Rabbit pAb(bs-0269R)-50ul/100ul/200ul
    ¥1180 - 2800