Sample:
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Lymph node (Mouse) Lysate at 40 ug
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Primary: Anti- Cytochrome P450 1A1 + 1A2 (bs-14174R) at 1/1000 dilution
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Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
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Predicted band size: 58 kD
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Observed band size: 58 kD
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- ￥1180 - 2800
- Bioss
- bs-14174R
- 2026年10月13日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-14174R |
| 英文名称 | Cytochrome P450 1A1 + 1A2 Rabbit pAb |
| 中文名称 | 细胞色素P450 1A1+1A2抗体 |
| 英文别名 | Aryl hydrocarbon hydroxylase; CP11; CP12; CYP1A1; CYP1A2; CYPIA1; CP1A1_HUMAN; CYPIA2; CP1A2_HUMAN; Cytochrome P450 family 1 subfamily A polypeptide 1; Cytochrome P450 family 1 subfamily A polypeptide 2; Cytochrome P450 subfamily I aromatic compound inducible polypeptide 1; Cytochrome P450 subfamily I aromatic compound inducible polypeptide 2; P1 450; P3 450; P450 form 4; P450 form 6; P450 P1; P450 P3. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Mouse (Human, Rat, Dog, Cow, Rabbit, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Cytochrome P450 1A1 + 1A2 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 58 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cancer Metabolism > Cellular metabolic process Cancer > Cancer Metabolism > Response to hypoxia Cardiovascular > Lipids / Lipoproteins > Lipid Metabolism > Cytochromes Metabolism > Pathways and Processes > Metabolic signaling pathways > Drug metabolism Metabolism > Pathways and Processes > Metabolic signaling pathways > Lipid and lipoprotein metabolism > Lipases Metabolism > Pathways and Processes > Metabolism processes > Hypoxia Metabolism > Pathways and Processes > Mitochondrial Metabolism > Cytochromes Metabolism > Types of disease > Cancer Signal Transduction > Metabolism > Drug metabolism |
| 亚细胞定位 | Endoplasmic reticulum |
| 组织特异性 | Lung, lymphocytes and placenta. |
| 相似性 | Belongs to the cytochrome P450 family. |
| 功能 | Cytochrome P450 oxidase (commonly abbreviated CYP) is a generic term for a large number of related, but distinct, oxidative enzymes important in vertebrate physiology. The cytochrome P450 mixed-function monooxygenase system is probably the most important element of Phase I metabolism in mammals. P450s are membrane-bound, either in the inner membrane of mitochondria or in the endoplasmic reticulum of cells where they metabolise thousands of endogenous and exogenous compounds. In the liver, these substrates include toxins, drugs, and other unneeded and potentially harmful molecules. Humans have 18 families of cytochrome P450 genes and 43 subfamilies; the CYP1 family is involved in drug metabolism and includes 3 subfamilies, 3 genes and 1 pseudogene. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene, CYP1A1, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. The gene has been associated with lung cancer risk. A related family member, CYP1A2, is located approximately 25 kb away from CYP1A1 on chromosome 15. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
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文献和实验该产品被引用文献
[IF=4.8] Xuwen Mao. et al. Mechanistic study on the restoration of intestinal barrier integrity and alleviation of inflammatory bowel disease by rhynchophylline via the AhR-NR4A1 pathway. FRONT PHARMACOL. 2025 Sep;16: WB Mouse
相关实验
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
Steady State ATPase Assays Coupled Enzyme System
DDW 10 mM Mg・ATP 30 mM PEP 6 mM NADH (Make fresh in 10 mM Tris-OAc pH 7.5, 4.26 mg/mL = 6 mM) PK/LDH (Sigma #P-0294, PK + LDH
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
Immunostaining 24. Wash stored slides twice in PBS (10 min each wash). 25. Wash slides once in PBS containing 1% Triton X-100 for 10 min. 26. Incubate slides in a slide jar containing blocking solution for 1 h
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CYP1A1&CYP1A2 Rabbit pAb(bs-14174R)-50ul/100ul/200ul
￥1180 - 2800
