GPER1 | GPR30 Rabbit pAb(bs-1380R)-50ul/100ul/200ul产品图
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GPER1 | GPR30 Rabbit pAb(bs-13

80R)-50ul/100ul/200ul
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  • ¥1180 - 2800
  • Bioss已认证
  • bs-1380R
  • 2026年10月13日
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      50ul/100ul/200ul

    规格:50ul产品价格:¥1180.0
    规格:100ul产品价格:¥1980.0
    规格:200ul产品价格:¥2800.0
    产品编号bs-1380R
    英文名称GPR30 Rabbit pAb
    中文名称G蛋白偶联受体30抗体
    英文别名G Protein Coupled Receptor 30; G-protein coupled receptor 30; G-protein coupled estrogen receptor 1; Membrane estrogen receptor; mER; Chemokine receptor-like 2; IL8-related receptor DRY12; Flow-induced endothelial G-protein coupled receptor 1; FEG-1; Lymphocyte-derived G-protein coupled receptor; LYGPR; GPCR-BR; CEPR; CMKRL2; DRY12; GPER.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse, Rat
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human GPR30
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量42 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Epigenetics and Nuclear Signaling > Nuclear Signaling Pathways > Nuclear Receptors > Estrogen

    Metabolism > Pathways and Processes > Endocrine metabolism > Hormone biosynthesis

    Metabolism > Pathways and Processes > Metabolic signaling pathways > Energy transfer pathways > Energy Metabolism

    Neuroscience > Neurotransmission > Receptors / Channels > GPCR > More GPCR

    Signal Transduction > Signaling Pathway > G Protein Signaling > GPCR

    亚细胞定位Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). Golgi apparatus membrane; Multi-pass membrane protein (By similarity). Note=Protein has been detected in the cell membrane, endoplasmic reticulum and Golgi apparatus. It is currently unclear whether this is a cell surface or intracellular receptor.
    组织特异性Ubiquitously expressed, but is most abundant in placenta. In brain regions, expressed as a 2.8 kb transcript in basal forebrain, frontal cortex, thalamus, hippocampus, caudate and putamen.
    相似性Belongs to the G-protein coupled receptor 1 family.
    功能Receptor for estrogen.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料This gene is a member of the G-protein coupled receptor 1 family and encodes a multi-pass membrane protein that localizes to the endoplasmic reticulum. The protein binds estrogen, resulting in intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5-trisphosphate in the nucleus. This protein therefore plays a role in the rapid nongenomic signaling events widely observed following stimulation of cells and tissues with estrogen. Alternate transcriptional splice variants which encode the same protein have been characterized. [provided by RefSeq, Jul 2008]

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}
    {Flow-Cyt}{1μg/Test}

     

    产品细节图片1
    25 ug total protein per lane of various lysates (see on figure) probed with GPR30 polyclonal antibody, unconjugated (bs-1380R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
    产品细节图片2
    P‌‌araformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GPR30 Polyclonal Antibody, Unconjugated (bs-1380R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    产品细节图片3
    P‌‌araformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GPR30 Polyclonal Antibody, Unconjugated (bs-1380R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    产品细节图片4
    P‌‌araformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GPR30 Polyclonal Antibody, Unconjugated (bs-1380R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    产品细节图片5
    P‌‌araformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GPR30 Polyclonal Antibody, Unconjugated (bs-1380R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    产品细节图片6
    Blank control (blue line): A431 cells (fixed with 70% methanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃).
    _x000D_ Primary Antibody (green line): Rabbit Anti-PR30 antibody (bs-1380R),Dilution: 1μg /10^6 cells;
    _x000D_ Isotype Control Antibody (orange line): Rabbit IgG .
    _x000D_ Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.

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    GPER1 | GPR30 Rabbit pAb(bs-1380R)-50ul/100ul/200ul
    ¥1180 - 2800